Magnetofection offers spatial targeting for anti-tumour gene therapies, which are an underexploited means of improving the standard of cancer treatment. This thesis examined novel combinations of several existing high performing non-viral transfection agents with magnetic moieties to achieve superior gene delivery when effected by oscillating magnet arrays. PEI-PGMA nanospheres with embedded SPIONs, and dendronised HEMA- GMA co-polymer electrostatically bound to SPIONs were unpromising magnetofection agents. However, association of nTMag Plus to Lipofectamine 2000 improved its transfection efficiency 2-fold for transfection times of 1 hour and less, when magnets oscillated at 2Hz across 0.22mm.
|12 Jul 2018
|Unpublished - 2018