Observations of novel magnetofection agents, and implications for magnetofection agent design and assessment

Roland Kerr

Research output: ThesisMaster's Thesis

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Abstract

Magnetofection offers spatial targeting for anti-tumour gene therapies, which are an underexploited means of improving the standard of cancer treatment. This thesis examined novel combinations of several existing high performing non-viral transfection agents with magnetic moieties to achieve superior gene delivery when effected by oscillating magnet arrays. PEI-PGMA nanospheres with embedded SPIONs, and dendronised HEMA- GMA co-polymer electrostatically bound to SPIONs were unpromising magnetofection agents. However, association of nTMag Plus to Lipofectamine 2000 improved its transfection efficiency 2-fold for transfection times of 1 hour and less, when magnets oscillated at 2Hz across 0.22mm.
Original languageEnglish
QualificationMasters
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Swaminatha Iyer, Killugudi, Supervisor
  • Evans, Cameron, Supervisor
  • Saunders, Martin, Supervisor
  • St Pierre, Tim, Supervisor
Thesis sponsors
Award date12 Jul 2018
DOIs
Publication statusUnpublished - 2018

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