Abstract
Analysis of immunohistochemical expression is often a subjective and semiquantitative process that can lead to the inconsistent reporting of results. To assess the effect that region selection and quantification method have on results, five different cancer stem cell markers were used in this study to compare tissue scoring with digital analysis methods that used three different tissue annotation methods. Samples of tumour and normal mucosa were used from 10 consecutive stage II colon cancer patients and stained for the putative cancer stem cell markers ALDH1, CD44v6, CD133, Lgr5 and SOX2. Tissue scoring was found to have considerably different results to digital analysis with the three different digital methods harbouring concordant results overall. However, SOX2 on normal tissue and CD133 on tumour and normal tissue produced discordant results which could be attributed to the different regions of tissue that were analysed. It is important that quantification method and selection of analysis areas are considered as part of study design to ensure that reproducible and consistent results are reported in the literature.
| Original language | English |
|---|---|
| Pages (from-to) | 24-29 |
| Number of pages | 6 |
| Journal | Pathology |
| Volume | 49 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2017 |
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Objective analysis of cancer stem cell marker expression using immunohistochemistry. / Miller, T. J.; McCoy, M. J.; Hemmings, C.; Bulsara, M. K.; Iacopetta, B.; Platell, C. F.
In: Pathology, Vol. 49, No. 1, 01.01.2017, p. 24-29.Research output: Contribution to journal › Article
TY - JOUR
T1 - Objective analysis of cancer stem cell marker expression using immunohistochemistry
AU - Miller, T. J.
AU - McCoy, M. J.
AU - Hemmings, C.
AU - Bulsara, M. K.
AU - Iacopetta, B.
AU - Platell, C. F.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Analysis of immunohistochemical expression is often a subjective and semiquantitative process that can lead to the inconsistent reporting of results. To assess the effect that region selection and quantification method have on results, five different cancer stem cell markers were used in this study to compare tissue scoring with digital analysis methods that used three different tissue annotation methods. Samples of tumour and normal mucosa were used from 10 consecutive stage II colon cancer patients and stained for the putative cancer stem cell markers ALDH1, CD44v6, CD133, Lgr5 and SOX2. Tissue scoring was found to have considerably different results to digital analysis with the three different digital methods harbouring concordant results overall. However, SOX2 on normal tissue and CD133 on tumour and normal tissue produced discordant results which could be attributed to the different regions of tissue that were analysed. It is important that quantification method and selection of analysis areas are considered as part of study design to ensure that reproducible and consistent results are reported in the literature.
AB - Analysis of immunohistochemical expression is often a subjective and semiquantitative process that can lead to the inconsistent reporting of results. To assess the effect that region selection and quantification method have on results, five different cancer stem cell markers were used in this study to compare tissue scoring with digital analysis methods that used three different tissue annotation methods. Samples of tumour and normal mucosa were used from 10 consecutive stage II colon cancer patients and stained for the putative cancer stem cell markers ALDH1, CD44v6, CD133, Lgr5 and SOX2. Tissue scoring was found to have considerably different results to digital analysis with the three different digital methods harbouring concordant results overall. However, SOX2 on normal tissue and CD133 on tumour and normal tissue produced discordant results which could be attributed to the different regions of tissue that were analysed. It is important that quantification method and selection of analysis areas are considered as part of study design to ensure that reproducible and consistent results are reported in the literature.
KW - ALDH1
KW - cancer stem cell
KW - CD133
KW - CD44v6
KW - Colorectal cancer
KW - immunohistochemistry
KW - Lgr5
KW - SOX2
UR - http://www.scopus.com/inward/record.url?scp=85006483474&partnerID=8YFLogxK
U2 - 10.1016/j.pathol.2016.09.063
DO - 10.1016/j.pathol.2016.09.063
M3 - Article
VL - 49
SP - 24
EP - 29
JO - Pathology
JF - Pathology
SN - 0031-3025
IS - 1
ER -