NT-4/5 reduces naturally occurring retinal ganglion cell death in neonatal rats

Q. Cui, Alan Harvey

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

THE retrograde nucleophilic tracer diamidino yellow (DY) was injected into the left superior colliculi of 2-day-old rats. Two days later, right eyes were injected with either neurotrophin NT-4/5, cycloheximide (CHX), MK-801 and DNQX (glutamate receptor antagonists), or saline. Almost all rats were perfused 6-7 h later and the numbers of normal and pyknotic DY-labelled retinal ganglion cells (RGCs) were determined from retinal whole mounts. In controls (no eye injection) the proportion of pyknotic RGCs was 1.04%. This level of naturally occurring death was significantly reduced after injection of NT-4/5 (0.34%); normal RGC density was also higher in this group. RGC pyknosis was increased after saline (1.21%), MK-801/DNQX (1.22%) or CHX (1.48%) injections but only in the latter case was the increase significantly greater than control.
Original languageEnglish
Pages (from-to)1882-1884
JournalNeuroReport
Volume5
Issue number15
DOIs
Publication statusPublished - 1994

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Retinal Ganglion Cells
Cell Death
Dizocilpine Maleate
Cycloheximide
Injections
Excitatory Amino Acid Antagonists
Superior Colliculi
Cell Count
neurotrophin 4
FG 9041
diamidino yellow

Cite this

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title = "NT-4/5 reduces naturally occurring retinal ganglion cell death in neonatal rats",
abstract = "THE retrograde nucleophilic tracer diamidino yellow (DY) was injected into the left superior colliculi of 2-day-old rats. Two days later, right eyes were injected with either neurotrophin NT-4/5, cycloheximide (CHX), MK-801 and DNQX (glutamate receptor antagonists), or saline. Almost all rats were perfused 6-7 h later and the numbers of normal and pyknotic DY-labelled retinal ganglion cells (RGCs) were determined from retinal whole mounts. In controls (no eye injection) the proportion of pyknotic RGCs was 1.04{\%}. This level of naturally occurring death was significantly reduced after injection of NT-4/5 (0.34{\%}); normal RGC density was also higher in this group. RGC pyknosis was increased after saline (1.21{\%}), MK-801/DNQX (1.22{\%}) or CHX (1.48{\%}) injections but only in the latter case was the increase significantly greater than control.",
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NT-4/5 reduces naturally occurring retinal ganglion cell death in neonatal rats. / Cui, Q.; Harvey, Alan.

In: NeuroReport, Vol. 5, No. 15, 1994, p. 1882-1884.

Research output: Contribution to journalArticle

TY - JOUR

T1 - NT-4/5 reduces naturally occurring retinal ganglion cell death in neonatal rats

AU - Cui, Q.

AU - Harvey, Alan

PY - 1994

Y1 - 1994

N2 - THE retrograde nucleophilic tracer diamidino yellow (DY) was injected into the left superior colliculi of 2-day-old rats. Two days later, right eyes were injected with either neurotrophin NT-4/5, cycloheximide (CHX), MK-801 and DNQX (glutamate receptor antagonists), or saline. Almost all rats were perfused 6-7 h later and the numbers of normal and pyknotic DY-labelled retinal ganglion cells (RGCs) were determined from retinal whole mounts. In controls (no eye injection) the proportion of pyknotic RGCs was 1.04%. This level of naturally occurring death was significantly reduced after injection of NT-4/5 (0.34%); normal RGC density was also higher in this group. RGC pyknosis was increased after saline (1.21%), MK-801/DNQX (1.22%) or CHX (1.48%) injections but only in the latter case was the increase significantly greater than control.

AB - THE retrograde nucleophilic tracer diamidino yellow (DY) was injected into the left superior colliculi of 2-day-old rats. Two days later, right eyes were injected with either neurotrophin NT-4/5, cycloheximide (CHX), MK-801 and DNQX (glutamate receptor antagonists), or saline. Almost all rats were perfused 6-7 h later and the numbers of normal and pyknotic DY-labelled retinal ganglion cells (RGCs) were determined from retinal whole mounts. In controls (no eye injection) the proportion of pyknotic RGCs was 1.04%. This level of naturally occurring death was significantly reduced after injection of NT-4/5 (0.34%); normal RGC density was also higher in this group. RGC pyknosis was increased after saline (1.21%), MK-801/DNQX (1.22%) or CHX (1.48%) injections but only in the latter case was the increase significantly greater than control.

U2 - 10.1097/00001756-199410000-00010

DO - 10.1097/00001756-199410000-00010

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SN - 0959-4965

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