Research output per year
Research output per year
Han Leng Ng, Rhonda L. Taylor, Jessica Cheng, Lawrence J. Abraham, Elizabeth Quail, Mark N. Cruickshank, Daniela Ulgiati
Research output: Contribution to journal › Article › peer-review
During mammalian lymphoid development, Notch signaling is necessary at multiple stages of T lymphopoiesis, including lineage commitment, and later stages of T cell effector differentiation. In contrast, outside of a defined role in the development of splenic marginal zone B cells, there is conflicting evidence regarding whether Notch signaling plays functional roles in other B cell sub-populations. Complement receptor 2 (CR2) modulates BCR-signaling and is tightly regulated throughout differentiation. During B lymphopoiesis, CR2 is detected on immature and mature B cells with high surface expression on marginal zone B cells. Here, we have explored the possibility that Notch regulates human CR2 transcriptional activity using in vitro models including a co-culture system, co-transfection gene reporters and chromatin accessibility assays. We provide evidence that Notch signaling regulates CR2 promoter activity in a mature B cell line, as well as the induction of endogenous CR2 mRNA in a non-expressing pre-B cell line. The dynamics of endogenous gene activation suggests additional unidentified factors are required to mediate surface CR2 expression on immature and mature B lineage cells.
Original language | English |
---|---|
Pages (from-to) | 150-164 |
Number of pages | 15 |
Journal | Molecular Immunology |
Volume | 128 |
DOIs | |
Publication status | Published - Dec 2020 |
Research output: Thesis › Doctoral Thesis