Nondestructive phenomic tools for the prediction of heat and drought tolerance at anthesis in Brassica Species

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Abstract

Oilseed Brassica species are vulnerable to heat and droughtstress, especially in the early reproductive stage. We evaluated plant imagingof whole plant and flower tissue, leaf stomatal conductance, leaf and budtemperature, photochemical reflectance index, quantum yield of photosynthesis,and leaf gas exchange for their suitability to detect tolerance to heat (H)and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicatedfactorial experiment was set up with 7 d of stress treatment from the beginningof anthesis with various levels of three factors H, D, and G. Most phenomicstools detected plant stress as indicated by significant main effects of H, D,and H×D. Whole plant volume was highly correlated with fresh weight changes,suggesting that whole plant imaging may be a useful surrogate for fresh weightin future studies. Vcmax, the maximum carboxylation rate of photosynthesis,increased rapidly on day 1 in H and H+D treatments, and there were significantinteractions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+Dtreatments was positively correlated with their harvested seed yield. Vcmax onday 1 and day 3 were clustered with seed yield in H and H+D treatments as shownin the heatmaps of genotypic correlations. TPU, the rate of triose phosphateuse, also showed significant positive genotypic correlations with seed yield inH+D treatments. Flower volume showed significant interactions of G×H and G×D onday 7, and flower volume of genotypes on day 7 in H was positively correlatedwith their harvested seed yield. There were few interactions of G×H or G×D forleaf stomatal conductance, leaf and bud temperature, photochemical reflectanceindex, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowersare potential nondestructive phenomic traits for heat or combined heat anddrought stress tolerance screening in Brassica germplasm. 

Original languageEnglish
Article number3264872
Number of pages16
JournalPlant Phenomics
Volume2019
DOIs
Publication statusPublished - 22 May 2019

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Brassica
heat tolerance
drought tolerance
seed yield
flowering
prediction
photosynthesis
flowers
heat
stomatal conductance
genotype
leaves
trioses
plant stress
carboxylation
oilseeds
stress tolerance
reflectance
gas exchange
heat stress

Cite this

@article{593058a6430142eeb5300bb2929e940c,
title = "Nondestructive phenomic tools for the prediction of heat and drought tolerance at anthesis in Brassica Species",
abstract = "Oilseed Brassica species are vulnerable to heat and droughtstress, especially in the early reproductive stage. We evaluated plant imagingof whole plant and flower tissue, leaf stomatal conductance, leaf and budtemperature, photochemical reflectance index, quantum yield of photosynthesis,and leaf gas exchange for their suitability to detect tolerance to heat (H)and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicatedfactorial experiment was set up with 7 d of stress treatment from the beginningof anthesis with various levels of three factors H, D, and G. Most phenomicstools detected plant stress as indicated by significant main effects of H, D,and H×D. Whole plant volume was highly correlated with fresh weight changes,suggesting that whole plant imaging may be a useful surrogate for fresh weightin future studies. Vcmax, the maximum carboxylation rate of photosynthesis,increased rapidly on day 1 in H and H+D treatments, and there were significantinteractions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+Dtreatments was positively correlated with their harvested seed yield. Vcmax onday 1 and day 3 were clustered with seed yield in H and H+D treatments as shownin the heatmaps of genotypic correlations. TPU, the rate of triose phosphateuse, also showed significant positive genotypic correlations with seed yield inH+D treatments. Flower volume showed significant interactions of G×H and G×D onday 7, and flower volume of genotypes on day 7 in H was positively correlatedwith their harvested seed yield. There were few interactions of G×H or G×D forleaf stomatal conductance, leaf and bud temperature, photochemical reflectanceindex, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowersare potential nondestructive phenomic traits for heat or combined heat anddrought stress tolerance screening in Brassica germplasm. ",
author = "Sheng Chen and Yiming Guo and Xavier Sirault and Katia Stefanova and Renu Saradadevi and Neil Turner and Matthew Nelson and Furbank, {Robert T} and Kadambot Siddique and Wallace Cowling",
year = "2019",
month = "5",
day = "22",
doi = "10.34133/2019/3264872",
language = "English",
volume = "2019",
journal = "Plant Phenomics",
issn = "2643-6515",
publisher = "American Association for the Advancement of Science (AAAS)",

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TY - JOUR

T1 - Nondestructive phenomic tools for the prediction of heat and drought tolerance at anthesis in Brassica Species

AU - Chen, Sheng

AU - Guo, Yiming

AU - Sirault, Xavier

AU - Stefanova, Katia

AU - Saradadevi, Renu

AU - Turner, Neil

AU - Nelson, Matthew

AU - Furbank, Robert T

AU - Siddique, Kadambot

AU - Cowling, Wallace

PY - 2019/5/22

Y1 - 2019/5/22

N2 - Oilseed Brassica species are vulnerable to heat and droughtstress, especially in the early reproductive stage. We evaluated plant imagingof whole plant and flower tissue, leaf stomatal conductance, leaf and budtemperature, photochemical reflectance index, quantum yield of photosynthesis,and leaf gas exchange for their suitability to detect tolerance to heat (H)and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicatedfactorial experiment was set up with 7 d of stress treatment from the beginningof anthesis with various levels of three factors H, D, and G. Most phenomicstools detected plant stress as indicated by significant main effects of H, D,and H×D. Whole plant volume was highly correlated with fresh weight changes,suggesting that whole plant imaging may be a useful surrogate for fresh weightin future studies. Vcmax, the maximum carboxylation rate of photosynthesis,increased rapidly on day 1 in H and H+D treatments, and there were significantinteractions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+Dtreatments was positively correlated with their harvested seed yield. Vcmax onday 1 and day 3 were clustered with seed yield in H and H+D treatments as shownin the heatmaps of genotypic correlations. TPU, the rate of triose phosphateuse, also showed significant positive genotypic correlations with seed yield inH+D treatments. Flower volume showed significant interactions of G×H and G×D onday 7, and flower volume of genotypes on day 7 in H was positively correlatedwith their harvested seed yield. There were few interactions of G×H or G×D forleaf stomatal conductance, leaf and bud temperature, photochemical reflectanceindex, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowersare potential nondestructive phenomic traits for heat or combined heat anddrought stress tolerance screening in Brassica germplasm. 

AB - Oilseed Brassica species are vulnerable to heat and droughtstress, especially in the early reproductive stage. We evaluated plant imagingof whole plant and flower tissue, leaf stomatal conductance, leaf and budtemperature, photochemical reflectance index, quantum yield of photosynthesis,and leaf gas exchange for their suitability to detect tolerance to heat (H)and/or drought (D) stress treatments in 12 Brassica genotypes (G). A replicatedfactorial experiment was set up with 7 d of stress treatment from the beginningof anthesis with various levels of three factors H, D, and G. Most phenomicstools detected plant stress as indicated by significant main effects of H, D,and H×D. Whole plant volume was highly correlated with fresh weight changes,suggesting that whole plant imaging may be a useful surrogate for fresh weightin future studies. Vcmax, the maximum carboxylation rate of photosynthesis,increased rapidly on day 1 in H and H+D treatments, and there were significantinteractions of G×H and G×D. Vcmax of genotypes on day 1 in H and H+Dtreatments was positively correlated with their harvested seed yield. Vcmax onday 1 and day 3 were clustered with seed yield in H and H+D treatments as shownin the heatmaps of genotypic correlations. TPU, the rate of triose phosphateuse, also showed significant positive genotypic correlations with seed yield inH+D treatments. Flower volume showed significant interactions of G×H and G×D onday 7, and flower volume of genotypes on day 7 in H was positively correlatedwith their harvested seed yield. There were few interactions of G×H or G×D forleaf stomatal conductance, leaf and bud temperature, photochemical reflectanceindex, and quantum yield of photosynthesis. Vcmax, TPU, and volume of flowersare potential nondestructive phenomic traits for heat or combined heat anddrought stress tolerance screening in Brassica germplasm. 

U2 - 10.34133/2019/3264872

DO - 10.34133/2019/3264872

M3 - Article

VL - 2019

JO - Plant Phenomics

JF - Plant Phenomics

SN - 2643-6515

M1 - 3264872

ER -