No evidence of sperm conjugate formation in an Australian mouse bearing sperm with three hooks

Renee Firman, Blair Bentley, F. Bowman, Fernando Marchant, J. Parthenay, Jess Sawyer, Tom Stewart, James O'Shea

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sperm conjugation occurs when two or more sperm physically unite for motility or transport through the female reproductive tract. In many muroid rodent species, sperm conjugates have been shown to form by a single, conspicuous apical hook located on the sperm head. These sperm "trains" have been reported to be highly variable in size and, despite all the heads pointing in roughly the same direction, exhibit a relatively disordered arrangement. In some species, sperm "trains" have been shown to enhance sperm swimming speed, and thus have been suggested to be advantageous in sperm competition. Here, we assessed the behavior of sperm in the sandy inland mouse (Pseudomys hermannsburgensis), a muroid rodent that bears sperm with three apical hooks. First, we accrued genetic evidence of multiple paternity within "wild" litters to unequivocally show that sperm competition does occur in this species. Following this we utilized both in vitro and in vivo methodologies to determine whether sandy inland mouse sperm conjugate to form motile trains. Our observations of in vitro preparations of active sperm revealed that sandy inland mouse sperm exhibit rapid, progressive motility as individual cells only. Similarly, histological sections of the reproductive tracts of mated females revealed no in vivo evidence of sperm conjugate formation. We conclude that the unique, three-hooked morphology of the sandy inland mouse sperm does not facilitate the formation of motile conjugates, and discuss our findings in relation to the different hypotheses for the evolution of the muroid rodent hook/s. © 2013 The Authors. Ecology and Evolution.
Original languageEnglish
Pages (from-to)1856-1863
JournalEcology and Evolution
Volume3
Issue number7
DOIs
Publication statusPublished - 2013

Fingerprint

sperm
spermatozoa
mice
rodent
train
sperm competition
rodents
motility
paternity
litter
ecology

Cite this

Firman, Renee ; Bentley, Blair ; Bowman, F. ; Marchant, Fernando ; Parthenay, J. ; Sawyer, Jess ; Stewart, Tom ; O'Shea, James. / No evidence of sperm conjugate formation in an Australian mouse bearing sperm with three hooks. In: Ecology and Evolution. 2013 ; Vol. 3, No. 7. pp. 1856-1863.
@article{dc9a0b26249340caa55630ea2f17d22d,
title = "No evidence of sperm conjugate formation in an Australian mouse bearing sperm with three hooks",
abstract = "Sperm conjugation occurs when two or more sperm physically unite for motility or transport through the female reproductive tract. In many muroid rodent species, sperm conjugates have been shown to form by a single, conspicuous apical hook located on the sperm head. These sperm {"}trains{"} have been reported to be highly variable in size and, despite all the heads pointing in roughly the same direction, exhibit a relatively disordered arrangement. In some species, sperm {"}trains{"} have been shown to enhance sperm swimming speed, and thus have been suggested to be advantageous in sperm competition. Here, we assessed the behavior of sperm in the sandy inland mouse (Pseudomys hermannsburgensis), a muroid rodent that bears sperm with three apical hooks. First, we accrued genetic evidence of multiple paternity within {"}wild{"} litters to unequivocally show that sperm competition does occur in this species. Following this we utilized both in vitro and in vivo methodologies to determine whether sandy inland mouse sperm conjugate to form motile trains. Our observations of in vitro preparations of active sperm revealed that sandy inland mouse sperm exhibit rapid, progressive motility as individual cells only. Similarly, histological sections of the reproductive tracts of mated females revealed no in vivo evidence of sperm conjugate formation. We conclude that the unique, three-hooked morphology of the sandy inland mouse sperm does not facilitate the formation of motile conjugates, and discuss our findings in relation to the different hypotheses for the evolution of the muroid rodent hook/s. {\circledC} 2013 The Authors. Ecology and Evolution.",
author = "Renee Firman and Blair Bentley and F. Bowman and Fernando Marchant and J. Parthenay and Jess Sawyer and Tom Stewart and James O'Shea",
year = "2013",
doi = "10.1002/ece3.577",
language = "English",
volume = "3",
pages = "1856--1863",
journal = "Ecology and Evolution",
issn = "2045-7758",
publisher = "John Wiley & Sons",
number = "7",

}

No evidence of sperm conjugate formation in an Australian mouse bearing sperm with three hooks. / Firman, Renee; Bentley, Blair; Bowman, F.; Marchant, Fernando; Parthenay, J.; Sawyer, Jess; Stewart, Tom; O'Shea, James.

In: Ecology and Evolution, Vol. 3, No. 7, 2013, p. 1856-1863.

Research output: Contribution to journalArticle

TY - JOUR

T1 - No evidence of sperm conjugate formation in an Australian mouse bearing sperm with three hooks

AU - Firman, Renee

AU - Bentley, Blair

AU - Bowman, F.

AU - Marchant, Fernando

AU - Parthenay, J.

AU - Sawyer, Jess

AU - Stewart, Tom

AU - O'Shea, James

PY - 2013

Y1 - 2013

N2 - Sperm conjugation occurs when two or more sperm physically unite for motility or transport through the female reproductive tract. In many muroid rodent species, sperm conjugates have been shown to form by a single, conspicuous apical hook located on the sperm head. These sperm "trains" have been reported to be highly variable in size and, despite all the heads pointing in roughly the same direction, exhibit a relatively disordered arrangement. In some species, sperm "trains" have been shown to enhance sperm swimming speed, and thus have been suggested to be advantageous in sperm competition. Here, we assessed the behavior of sperm in the sandy inland mouse (Pseudomys hermannsburgensis), a muroid rodent that bears sperm with three apical hooks. First, we accrued genetic evidence of multiple paternity within "wild" litters to unequivocally show that sperm competition does occur in this species. Following this we utilized both in vitro and in vivo methodologies to determine whether sandy inland mouse sperm conjugate to form motile trains. Our observations of in vitro preparations of active sperm revealed that sandy inland mouse sperm exhibit rapid, progressive motility as individual cells only. Similarly, histological sections of the reproductive tracts of mated females revealed no in vivo evidence of sperm conjugate formation. We conclude that the unique, three-hooked morphology of the sandy inland mouse sperm does not facilitate the formation of motile conjugates, and discuss our findings in relation to the different hypotheses for the evolution of the muroid rodent hook/s. © 2013 The Authors. Ecology and Evolution.

AB - Sperm conjugation occurs when two or more sperm physically unite for motility or transport through the female reproductive tract. In many muroid rodent species, sperm conjugates have been shown to form by a single, conspicuous apical hook located on the sperm head. These sperm "trains" have been reported to be highly variable in size and, despite all the heads pointing in roughly the same direction, exhibit a relatively disordered arrangement. In some species, sperm "trains" have been shown to enhance sperm swimming speed, and thus have been suggested to be advantageous in sperm competition. Here, we assessed the behavior of sperm in the sandy inland mouse (Pseudomys hermannsburgensis), a muroid rodent that bears sperm with three apical hooks. First, we accrued genetic evidence of multiple paternity within "wild" litters to unequivocally show that sperm competition does occur in this species. Following this we utilized both in vitro and in vivo methodologies to determine whether sandy inland mouse sperm conjugate to form motile trains. Our observations of in vitro preparations of active sperm revealed that sandy inland mouse sperm exhibit rapid, progressive motility as individual cells only. Similarly, histological sections of the reproductive tracts of mated females revealed no in vivo evidence of sperm conjugate formation. We conclude that the unique, three-hooked morphology of the sandy inland mouse sperm does not facilitate the formation of motile conjugates, and discuss our findings in relation to the different hypotheses for the evolution of the muroid rodent hook/s. © 2013 The Authors. Ecology and Evolution.

U2 - 10.1002/ece3.577

DO - 10.1002/ece3.577

M3 - Article

VL - 3

SP - 1856

EP - 1863

JO - Ecology and Evolution

JF - Ecology and Evolution

SN - 2045-7758

IS - 7

ER -