TY - JOUR
T1 - Next is now: new technologies for sequencing of genomes
AU - Lister, Ryan
AU - Gregory, B.D.
AU - Ecker, J.R.
PY - 2009
Y1 - 2009
N2 - The sudden availability of DNA sequencing technologies that rapidly produce vast amounts of sequence information has triggered a paradigm shift in genomics, enabling massively parallel surveying of complex nucleic acid populations. The diversity of applications to which these technologies have already been applied demonstrates the immense range of cellular processes and properties that can now be studied at the single-base resolution. These include genome resequencing and polymorphism discovery, mutation mapping, DNA methylation, histone modifications, transcriptome sequencing, gene discovery, alternative splicing identification, small RNA profiling, DNA-protein, and possibly even protein-protein interactions. Thus, these deep sequencing technologies offer plant biologists unprecedented opportunities to increase the understanding of the functions and dynamics of plant cells and populations. © 2008 Elsevier Ltd. All rights reserved.
AB - The sudden availability of DNA sequencing technologies that rapidly produce vast amounts of sequence information has triggered a paradigm shift in genomics, enabling massively parallel surveying of complex nucleic acid populations. The diversity of applications to which these technologies have already been applied demonstrates the immense range of cellular processes and properties that can now be studied at the single-base resolution. These include genome resequencing and polymorphism discovery, mutation mapping, DNA methylation, histone modifications, transcriptome sequencing, gene discovery, alternative splicing identification, small RNA profiling, DNA-protein, and possibly even protein-protein interactions. Thus, these deep sequencing technologies offer plant biologists unprecedented opportunities to increase the understanding of the functions and dynamics of plant cells and populations. © 2008 Elsevier Ltd. All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=62349102325&partnerID=8YFLogxK
U2 - 10.1016/j.pbi.2008.11.004
DO - 10.1016/j.pbi.2008.11.004
M3 - Review article
C2 - 19157957
SN - 1369-5266
VL - 12
SP - 107
EP - 118
JO - Current Opinion in Plant Biology
JF - Current Opinion in Plant Biology
ER -