Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications

    Research output: Chapter in Book/Conference paperOther chapter contribution

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    Abstract

    Next-generation sequencing (NGS) technologies using DNA, RNA, or methylation sequencing have impacted enormously on the life sciences. NGS is the choice for large-scale genomic and transcriptomic sequencing because of the high-throughput production and outputs of sequencing data in the gigabase range per instrument run and the lower cost compared to the traditional Sanger first-generation sequencing method. The vast amounts of data generated by NGS have broadened our understanding of structural and functional genomics through the concepts of “omics” ranging from basic genomics to integrated systeomics, providing new insight into the workings and meaning of genetic conservation and diversity of living things. NGS today is more than ever about how different organisms use genetic information and molecular biology to survive and reproduce with and without mutations, disease, and diversity within their population networks and changing environments. In this chapter, the advances, applications, and challenges of NGS are reviewed starting with a history of first-generation sequencing followed by the major NGS platforms, the bioinformatics issues confronting NGS data storage and analysis, and the impacts made in the fields of genetics, biology, agriculture, and medicine in the brave, new world of ”omics
    Original languageEnglish
    Title of host publicationNext Generation Sequencing - Advances, Applications and Challenges
    EditorsJerzy K Kulski
    Place of PublicationCroatia
    PublisherInTech
    Pages3-60
    ISBN (Print)978-953-51-2240-1
    DOIs
    Publication statusPublished - 14 Jan 2016

    Publication series

    NameBiochemistry, Genetics and Molecular Biology
    PublisherInTech

    Fingerprint

    Genomics
    History
    Biological Science Disciplines
    Information Storage and Retrieval
    Computational Biology
    Agriculture
    Methylation
    Molecular Biology
    Medicine
    RNA
    Technology
    Costs and Cost Analysis
    Mutation
    DNA
    Population

    Cite this

    Kulski, J. (2016). Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications. In J. K. Kulski (Ed.), Next Generation Sequencing - Advances, Applications and Challenges (pp. 3-60). (Biochemistry, Genetics and Molecular Biology). Croatia: InTech. https://doi.org/10.5772/61964
    Kulski, Jerzy. / Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications. Next Generation Sequencing - Advances, Applications and Challenges. editor / Jerzy K Kulski. Croatia : InTech, 2016. pp. 3-60 (Biochemistry, Genetics and Molecular Biology).
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    Kulski, J 2016, Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications. in JK Kulski (ed.), Next Generation Sequencing - Advances, Applications and Challenges. Biochemistry, Genetics and Molecular Biology, InTech, Croatia, pp. 3-60. https://doi.org/10.5772/61964

    Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications. / Kulski, Jerzy.

    Next Generation Sequencing - Advances, Applications and Challenges. ed. / Jerzy K Kulski. Croatia : InTech, 2016. p. 3-60 (Biochemistry, Genetics and Molecular Biology).

    Research output: Chapter in Book/Conference paperOther chapter contribution

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    Kulski J. Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications. In Kulski JK, editor, Next Generation Sequencing - Advances, Applications and Challenges. Croatia: InTech. 2016. p. 3-60. (Biochemistry, Genetics and Molecular Biology). https://doi.org/10.5772/61964