Abstract
The biological role of Arabidopsis nitrilases in plants is not well understood aside from their enzymatic role in detoxifying nitrile substrates. Identification and analysis of protein interactors for the four nitrilase isoforms through protein co-immunoprecipitation, database mining and Bimolecular Fluorescence Complementation showed a possible involvement of these enzymes in regulating biotic and abiotic stresses. Furthermore, it was shown that different nitrilase isoforms may have different roles in regulating biotic and abiotic stresses. Thus, this thesis indicates that the biological roles of nitrilases could be independent of their enzymatic roles, suggesting a secondary role in stress regulation.
Original language | English |
---|---|
Qualification | Doctor of Philosophy |
Awarding Institution |
|
Supervisors/Advisors |
|
Award date | 1 Nov 2016 |
Publication status | Unpublished - 2016 |