New findings in the fatty acid composition of individual platelet phospholipids in man after dietary fish oil supplementation

T A Mori, J P Codde, R Vandongen, L J Beilin

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Nine healthy male volunteers were given 15 Max EPA fish oil capsules providing 2.67 g of eicosapentaenoic acid (EPA, 20:5 omega 3) and 1.72 g of docosahexaenoic acid (DHA, 22:6 omega 3) daily for 3 wk. Measurements were taken at baseline, at the end of the fish-oil period, and at 2 and 6 wk postsupplementation. The effect of fish oil on plasma lipids and the fatty acid composition of individual platelet phospholipids was studied. In general, the proportions of 20:5 omega 3 and 22:6 omega 3 in platelet phosphoglycerides were substantially increased mainly at the expense of arachidonic acid (AA, 20:4 omega 6). A large and significant increase in the relative EPA content of phosphatidylcholine (PC) (P less than 0.001) and phosphatidylethanolamine (PE) (P less than 0.001) was noted at the end of the 3 wk supplementation. We have also shown for the first time a small but significant (P less than 0.001) incorporation of EPA in phosphatidylserine (PS). Incorporation of DHA was also detected in PC, PE and PS, whereas the relative AA content of these phospholipids was significantly reduced. Fish oil supplementation led to a significant increase of 22:5 omega 3 in PS and decreases of 20:3 omega 6 in PC and 22:4 omega 6 in PE. Postsupplementation measurements showed a gradual return of all fatty acids to baseline levels. The fatty acid composition of the phosphatidylinositol (PI) fraction remained unchanged throughout the trial period. We conclude that in humans omega 3 fatty acids are incorporated into platelet membrane phospholipid subclasses with a high degree of specificity.

Original languageEnglish
Pages (from-to)744-50
Number of pages7
JournalLipids
Volume22
Issue number10
Publication statusPublished - Oct 1987

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Unsaturated Dietary Fats
Fish Oils
Platelets
Phosphatidylserines
Phospholipids
Fatty Acids
Blood Platelets
Phosphatidylcholines
Chemical analysis
Glycerophospholipids
Eicosapentaenoic Acid
Docosahexaenoic Acids
Omega-3 Fatty Acids
Phosphatidylinositols
Arachidonic Acid
Capsules
Healthy Volunteers
Membranes
Lipids
Plasmas

Cite this

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title = "New findings in the fatty acid composition of individual platelet phospholipids in man after dietary fish oil supplementation",
abstract = "Nine healthy male volunteers were given 15 Max EPA fish oil capsules providing 2.67 g of eicosapentaenoic acid (EPA, 20:5 omega 3) and 1.72 g of docosahexaenoic acid (DHA, 22:6 omega 3) daily for 3 wk. Measurements were taken at baseline, at the end of the fish-oil period, and at 2 and 6 wk postsupplementation. The effect of fish oil on plasma lipids and the fatty acid composition of individual platelet phospholipids was studied. In general, the proportions of 20:5 omega 3 and 22:6 omega 3 in platelet phosphoglycerides were substantially increased mainly at the expense of arachidonic acid (AA, 20:4 omega 6). A large and significant increase in the relative EPA content of phosphatidylcholine (PC) (P less than 0.001) and phosphatidylethanolamine (PE) (P less than 0.001) was noted at the end of the 3 wk supplementation. We have also shown for the first time a small but significant (P less than 0.001) incorporation of EPA in phosphatidylserine (PS). Incorporation of DHA was also detected in PC, PE and PS, whereas the relative AA content of these phospholipids was significantly reduced. Fish oil supplementation led to a significant increase of 22:5 omega 3 in PS and decreases of 20:3 omega 6 in PC and 22:4 omega 6 in PE. Postsupplementation measurements showed a gradual return of all fatty acids to baseline levels. The fatty acid composition of the phosphatidylinositol (PI) fraction remained unchanged throughout the trial period. We conclude that in humans omega 3 fatty acids are incorporated into platelet membrane phospholipid subclasses with a high degree of specificity.",
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New findings in the fatty acid composition of individual platelet phospholipids in man after dietary fish oil supplementation. / Mori, T A; Codde, J P; Vandongen, R; Beilin, L J.

In: Lipids, Vol. 22, No. 10, 10.1987, p. 744-50.

Research output: Contribution to journalArticle

TY - JOUR

T1 - New findings in the fatty acid composition of individual platelet phospholipids in man after dietary fish oil supplementation

AU - Mori, T A

AU - Codde, J P

AU - Vandongen, R

AU - Beilin, L J

PY - 1987/10

Y1 - 1987/10

N2 - Nine healthy male volunteers were given 15 Max EPA fish oil capsules providing 2.67 g of eicosapentaenoic acid (EPA, 20:5 omega 3) and 1.72 g of docosahexaenoic acid (DHA, 22:6 omega 3) daily for 3 wk. Measurements were taken at baseline, at the end of the fish-oil period, and at 2 and 6 wk postsupplementation. The effect of fish oil on plasma lipids and the fatty acid composition of individual platelet phospholipids was studied. In general, the proportions of 20:5 omega 3 and 22:6 omega 3 in platelet phosphoglycerides were substantially increased mainly at the expense of arachidonic acid (AA, 20:4 omega 6). A large and significant increase in the relative EPA content of phosphatidylcholine (PC) (P less than 0.001) and phosphatidylethanolamine (PE) (P less than 0.001) was noted at the end of the 3 wk supplementation. We have also shown for the first time a small but significant (P less than 0.001) incorporation of EPA in phosphatidylserine (PS). Incorporation of DHA was also detected in PC, PE and PS, whereas the relative AA content of these phospholipids was significantly reduced. Fish oil supplementation led to a significant increase of 22:5 omega 3 in PS and decreases of 20:3 omega 6 in PC and 22:4 omega 6 in PE. Postsupplementation measurements showed a gradual return of all fatty acids to baseline levels. The fatty acid composition of the phosphatidylinositol (PI) fraction remained unchanged throughout the trial period. We conclude that in humans omega 3 fatty acids are incorporated into platelet membrane phospholipid subclasses with a high degree of specificity.

AB - Nine healthy male volunteers were given 15 Max EPA fish oil capsules providing 2.67 g of eicosapentaenoic acid (EPA, 20:5 omega 3) and 1.72 g of docosahexaenoic acid (DHA, 22:6 omega 3) daily for 3 wk. Measurements were taken at baseline, at the end of the fish-oil period, and at 2 and 6 wk postsupplementation. The effect of fish oil on plasma lipids and the fatty acid composition of individual platelet phospholipids was studied. In general, the proportions of 20:5 omega 3 and 22:6 omega 3 in platelet phosphoglycerides were substantially increased mainly at the expense of arachidonic acid (AA, 20:4 omega 6). A large and significant increase in the relative EPA content of phosphatidylcholine (PC) (P less than 0.001) and phosphatidylethanolamine (PE) (P less than 0.001) was noted at the end of the 3 wk supplementation. We have also shown for the first time a small but significant (P less than 0.001) incorporation of EPA in phosphatidylserine (PS). Incorporation of DHA was also detected in PC, PE and PS, whereas the relative AA content of these phospholipids was significantly reduced. Fish oil supplementation led to a significant increase of 22:5 omega 3 in PS and decreases of 20:3 omega 6 in PC and 22:4 omega 6 in PE. Postsupplementation measurements showed a gradual return of all fatty acids to baseline levels. The fatty acid composition of the phosphatidylinositol (PI) fraction remained unchanged throughout the trial period. We conclude that in humans omega 3 fatty acids are incorporated into platelet membrane phospholipid subclasses with a high degree of specificity.

KW - Adult

KW - Blood Platelets

KW - Dietary Fats

KW - Docosahexaenoic Acids

KW - Eicosapentaenoic Acid

KW - Fatty Acids

KW - Fish Oils

KW - Food, Fortified

KW - Humans

KW - Male

KW - Middle Aged

KW - Phosphatidylcholines

KW - Phospholipids

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 22

SP - 744

EP - 750

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 10

ER -