Neutralizing BDNF and FGF2 injection into denervated skeletal muscle improve recovery after nerve repair

Svenja Rink; Christina Chatziparaskeva; Luisa Elles; Stoyan Pavlov; Klaus Nohroudi; Habib Bendella; Levent Sarikcioglu; Marilena Manthou; Sarah Dunlop; Tessa Gordon; Doychin N. Angelov

doi:10.1002/mus.26991

Neutralizing BDNF and FGF2 injection into denervated skeletal muscle improve recovery after nerve repair

Svenja Rink, Christina Chatziparaskeva, Luisa Elles, Stoyan Pavlov, Klaus Nohroudi, Habib Bendella, Levent Sarikcioglu, Marilena Manthou, Sarah Dunlop, Tessa Gordon, Doychin N. Angelov

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Background: After facial nerve injury and surgical repair in rats, recovery of vibrissal whisking is associated with a high proportion of mono-innervated neuro-muscular junctions (NMJs). Our earlier work with Sprague Dawley (SD)/Royal College of Surgeons (RCS) rats, which are blind and spontaneously restore NMJ-monoinnervation and whisking, showed correlations between functional recovery and increase of fibroblast growth factor-2 (FGF2) and brain-derived neurotrophic factor (BDNF) in denervated vibrissal muscles. Methods: We used normally sighted rats (Wistar), in which NMJ-polyinnervation is highly correlated with poor whisking recovery, and injected the vibrissal muscle levator labii superioris (LLS) with combinations of BDNF, anti-BDNF, and FGF2 at different postoperative periods after facial nerve injury. Results: Rats receiving anti-BDNF+FGF2 showed low NMJ-polyinnervation and best recovery of whisking amplitude. Conclusions: Restoration of target reinnervation after peripheral nerve injury requires a complex mixture of trophic factors with a specific time course of availability for each of them.

Original language	English
Pages (from-to)	404-412
Number of pages	9
Journal	Muscle and Nerve
Volume	62
Issue number	3
DOIs	https://doi.org/10.1002/mus.26991
Publication status	Published - 1 Sept 2020

Access to Document

10.1002/mus.26991

Cite this

@article{1c8e5cf5b93e422b8d3d6df22ea9207e,

title = "Neutralizing BDNF and FGF2 injection into denervated skeletal muscle improve recovery after nerve repair",

abstract = "Background: After facial nerve injury and surgical repair in rats, recovery of vibrissal whisking is associated with a high proportion of mono-innervated neuro-muscular junctions (NMJs). Our earlier work with Sprague Dawley (SD)/Royal College of Surgeons (RCS) rats, which are blind and spontaneously restore NMJ-monoinnervation and whisking, showed correlations between functional recovery and increase of fibroblast growth factor-2 (FGF2) and brain-derived neurotrophic factor (BDNF) in denervated vibrissal muscles. Methods: We used normally sighted rats (Wistar), in which NMJ-polyinnervation is highly correlated with poor whisking recovery, and injected the vibrissal muscle levator labii superioris (LLS) with combinations of BDNF, anti-BDNF, and FGF2 at different postoperative periods after facial nerve injury. Results: Rats receiving anti-BDNF+FGF2 showed low NMJ-polyinnervation and best recovery of whisking amplitude. Conclusions: Restoration of target reinnervation after peripheral nerve injury requires a complex mixture of trophic factors with a specific time course of availability for each of them.",

keywords = "axotomy, BDNF, FGF2, functional recovery, motoneuron, motor endplates, polyinnervation, vibrissae whisking",

author = "Svenja Rink and Christina Chatziparaskeva and Luisa Elles and Stoyan Pavlov and Klaus Nohroudi and Habib Bendella and Levent Sarikcioglu and Marilena Manthou and Sarah Dunlop and Tessa Gordon and Angelov, {Doychin N.}",

year = "2020",

month = sep,

day = "1",

doi = "10.1002/mus.26991",

language = "English",

volume = "62",

pages = "404--412",

journal = "Muscle and Nerve",

issn = "0148-639X",

publisher = "John Wiley & Sons",

number = "3",

}

TY - JOUR

T1 - Neutralizing BDNF and FGF2 injection into denervated skeletal muscle improve recovery after nerve repair

AU - Rink, Svenja

AU - Chatziparaskeva, Christina

AU - Elles, Luisa

AU - Pavlov, Stoyan

AU - Nohroudi, Klaus

AU - Bendella, Habib

AU - Sarikcioglu, Levent

AU - Manthou, Marilena

AU - Dunlop, Sarah

AU - Gordon, Tessa

AU - Angelov, Doychin N.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Background: After facial nerve injury and surgical repair in rats, recovery of vibrissal whisking is associated with a high proportion of mono-innervated neuro-muscular junctions (NMJs). Our earlier work with Sprague Dawley (SD)/Royal College of Surgeons (RCS) rats, which are blind and spontaneously restore NMJ-monoinnervation and whisking, showed correlations between functional recovery and increase of fibroblast growth factor-2 (FGF2) and brain-derived neurotrophic factor (BDNF) in denervated vibrissal muscles. Methods: We used normally sighted rats (Wistar), in which NMJ-polyinnervation is highly correlated with poor whisking recovery, and injected the vibrissal muscle levator labii superioris (LLS) with combinations of BDNF, anti-BDNF, and FGF2 at different postoperative periods after facial nerve injury. Results: Rats receiving anti-BDNF+FGF2 showed low NMJ-polyinnervation and best recovery of whisking amplitude. Conclusions: Restoration of target reinnervation after peripheral nerve injury requires a complex mixture of trophic factors with a specific time course of availability for each of them.

AB - Background: After facial nerve injury and surgical repair in rats, recovery of vibrissal whisking is associated with a high proportion of mono-innervated neuro-muscular junctions (NMJs). Our earlier work with Sprague Dawley (SD)/Royal College of Surgeons (RCS) rats, which are blind and spontaneously restore NMJ-monoinnervation and whisking, showed correlations between functional recovery and increase of fibroblast growth factor-2 (FGF2) and brain-derived neurotrophic factor (BDNF) in denervated vibrissal muscles. Methods: We used normally sighted rats (Wistar), in which NMJ-polyinnervation is highly correlated with poor whisking recovery, and injected the vibrissal muscle levator labii superioris (LLS) with combinations of BDNF, anti-BDNF, and FGF2 at different postoperative periods after facial nerve injury. Results: Rats receiving anti-BDNF+FGF2 showed low NMJ-polyinnervation and best recovery of whisking amplitude. Conclusions: Restoration of target reinnervation after peripheral nerve injury requires a complex mixture of trophic factors with a specific time course of availability for each of them.

KW - axotomy

KW - BDNF

KW - FGF2

KW - functional recovery

KW - motoneuron

KW - motor endplates

KW - polyinnervation

KW - vibrissae whisking

UR - http://www.scopus.com/inward/record.url?scp=85087150929&partnerID=8YFLogxK

U2 - 10.1002/mus.26991

DO - 10.1002/mus.26991

M3 - Article

C2 - 32497302

AN - SCOPUS:85087150929

SN - 0148-639X

VL - 62

SP - 404

EP - 412

JO - Muscle and Nerve

JF - Muscle and Nerve

IS - 3

ER -

Neutralizing BDNF and FGF2 injection into denervated skeletal muscle improve recovery after nerve repair

Abstract

Access to Document

Other files and links

Fingerprint

Cite this