Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants

Suvi Honkanen, Anna Thamm, Mario A. Arteaga-Vazquez, Liam Dolan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Basic helix-loop-helix transcription factors encoded by RSL class I genes control a gene regulatory network that positively regulates the development of filamentous rooting cells -root hairs and rhizoids -in land plants. The GLABRA2 transcription factor negatively regulates these genes in the angiosperm Arabidopsis thaliana. To find negative regulators of RSL class I genes in early diverging land plants we conducted a mutant screen in the liverwort Marchantia polymorpha. This identified FEW RHIZOIDS1 (MpFRH1) microRNA (miRNA) that negatively regulates the RSL class I gene MpRSL1. The miRNA and its mRNA target constitute a feedback mechanism that controls epidermal cell differentiation. MpFRH1 miRNA target sites are conserved among liverwort RSL class I mRNAs but are not present in RSL class I mRNAs of other land plants. These findings indicate that while RSL class I genes are ancient and conserved, independent negative regulatory mechanisms evolved in different lineages during land plant evolution.

Original languageEnglish
Article numbere38529
JournaleLife
Volume7
DOIs
Publication statusPublished - 23 Aug 2018

Fingerprint

Embryophyta
MHC Class I Genes
Basic Helix-Loop-Helix Transcription Factors
Genes
MicroRNAs
Hepatophyta
Messenger RNA
Marchantia
Angiosperms
Gene Regulatory Networks
Arabidopsis
Cell Differentiation
Transcription Factors
Feedback

Cite this

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abstract = "Basic helix-loop-helix transcription factors encoded by RSL class I genes control a gene regulatory network that positively regulates the development of filamentous rooting cells -root hairs and rhizoids -in land plants. The GLABRA2 transcription factor negatively regulates these genes in the angiosperm Arabidopsis thaliana. To find negative regulators of RSL class I genes in early diverging land plants we conducted a mutant screen in the liverwort Marchantia polymorpha. This identified FEW RHIZOIDS1 (MpFRH1) microRNA (miRNA) that negatively regulates the RSL class I gene MpRSL1. The miRNA and its mRNA target constitute a feedback mechanism that controls epidermal cell differentiation. MpFRH1 miRNA target sites are conserved among liverwort RSL class I mRNAs but are not present in RSL class I mRNAs of other land plants. These findings indicate that while RSL class I genes are ancient and conserved, independent negative regulatory mechanisms evolved in different lineages during land plant evolution.",
author = "Suvi Honkanen and Anna Thamm and Arteaga-Vazquez, {Mario A.} and Liam Dolan",
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Negative regulation of conserved RSL class I bHLH transcription factors evolved independently among land plants. / Honkanen, Suvi; Thamm, Anna; Arteaga-Vazquez, Mario A.; Dolan, Liam.

In: eLife, Vol. 7, e38529, 23.08.2018.

Research output: Contribution to journalArticle

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AU - Honkanen, Suvi

AU - Thamm, Anna

AU - Arteaga-Vazquez, Mario A.

AU - Dolan, Liam

PY - 2018/8/23

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AB - Basic helix-loop-helix transcription factors encoded by RSL class I genes control a gene regulatory network that positively regulates the development of filamentous rooting cells -root hairs and rhizoids -in land plants. The GLABRA2 transcription factor negatively regulates these genes in the angiosperm Arabidopsis thaliana. To find negative regulators of RSL class I genes in early diverging land plants we conducted a mutant screen in the liverwort Marchantia polymorpha. This identified FEW RHIZOIDS1 (MpFRH1) microRNA (miRNA) that negatively regulates the RSL class I gene MpRSL1. The miRNA and its mRNA target constitute a feedback mechanism that controls epidermal cell differentiation. MpFRH1 miRNA target sites are conserved among liverwort RSL class I mRNAs but are not present in RSL class I mRNAs of other land plants. These findings indicate that while RSL class I genes are ancient and conserved, independent negative regulatory mechanisms evolved in different lineages during land plant evolution.

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