NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes

Cuiwen He, Xuchen Hu, Thomas A. Weston, Rachel S. Jung, Patrick Heizer, Yiping Tu, Rochelle Ellison, Ken Matsumoto, Holger Gerhardt, Peter Tontonoz, Loren G. Fong, Stephen G. Young, Haibo Jiang

Research output: Contribution to journalArticle

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Abstract

Heterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [2H]triglycerides (2H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined 13C enrichment in brown adipocytes after administering [13C]glucose or 13C-labeled mixed fatty acids by gastric gavage. The uptake of 2H-TRLs-derived lipids into brown adipocytes was heterogeneous, with 2H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in 13C enrichment in adjacent brown adipocytes after administering [13C]glucose or [13C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes.

Original languageEnglish
Pages (from-to)899-902
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume504
Issue number4
DOIs
Publication statusPublished - 12 Oct 2018

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Secondary Ion Mass Spectrometry
Brown Adipocytes
Secondary ion mass spectrometry
Nutrients
Tissue
Imaging techniques
Triglycerides
Adipocytes
Lipids
Fatty Acids
Brown Adipose Tissue
Glucose
Stomach
Lipoproteins
White Adipose Tissue
Intravenous Injections
Adipose Tissue
Food

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He, Cuiwen ; Hu, Xuchen ; Weston, Thomas A. ; Jung, Rachel S. ; Heizer, Patrick ; Tu, Yiping ; Ellison, Rochelle ; Matsumoto, Ken ; Gerhardt, Holger ; Tontonoz, Peter ; Fong, Loren G. ; Young, Stephen G. ; Jiang, Haibo. / NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 504, No. 4. pp. 899-902.
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He, C, Hu, X, Weston, TA, Jung, RS, Heizer, P, Tu, Y, Ellison, R, Matsumoto, K, Gerhardt, H, Tontonoz, P, Fong, LG, Young, SG & Jiang, H 2018, 'NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes' Biochemical and Biophysical Research Communications, vol. 504, no. 4, pp. 899-902. https://doi.org/10.1016/j.bbrc.2018.09.051

NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes. / He, Cuiwen; Hu, Xuchen; Weston, Thomas A.; Jung, Rachel S.; Heizer, Patrick; Tu, Yiping; Ellison, Rochelle; Matsumoto, Ken; Gerhardt, Holger; Tontonoz, Peter; Fong, Loren G.; Young, Stephen G.; Jiang, Haibo.

In: Biochemical and Biophysical Research Communications, Vol. 504, No. 4, 12.10.2018, p. 899-902.

Research output: Contribution to journalArticle

TY - JOUR

T1 - NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes

AU - He, Cuiwen

AU - Hu, Xuchen

AU - Weston, Thomas A.

AU - Jung, Rachel S.

AU - Heizer, Patrick

AU - Tu, Yiping

AU - Ellison, Rochelle

AU - Matsumoto, Ken

AU - Gerhardt, Holger

AU - Tontonoz, Peter

AU - Fong, Loren G.

AU - Young, Stephen G.

AU - Jiang, Haibo

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N2 - Heterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [2H]triglycerides (2H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined 13C enrichment in brown adipocytes after administering [13C]glucose or 13C-labeled mixed fatty acids by gastric gavage. The uptake of 2H-TRLs-derived lipids into brown adipocytes was heterogeneous, with 2H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in 13C enrichment in adjacent brown adipocytes after administering [13C]glucose or [13C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes.

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KW - Brown adipose tissue

KW - Fatty acids

KW - NanoSIMS imaging

KW - Nutrient uptake

KW - White adipose tissue

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DO - 10.1016/j.bbrc.2018.09.051

M3 - Article

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EP - 902

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