NanoSIMS chemical imaging combined with correlative microscopy for biological sample analysis

Haibo Jiang, Matthew R. Kilburn, Johan Decelle, Niculina Musat

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

Nano-scale Secondary Ion Mass Spectrometry (NanoSIMS) is one of the most powerful in situ elemental and isotopic analysis techniques available to biologists. The combination of stable isotope probing with NanoSIMS (nanoSIP) has opened up new avenues for biological studies over the past decade. However, due to limitations inherent with any analytical methodology, additional information from correlative techniques is usually required to address real biological questions. Here we review recent developments in correlative analysis applied to complex biological systems: first, high-resolution tracking of molecules (e.g. peptides, lipids) by correlation with electron microscopy and atomic force microscopy; second, identification of a specific microbial taxon with fluorescence in situ hybridization and quantification of its metabolic capacities; and, third, molecular specific imaging with new probes.

Original languageEnglish
Pages (from-to)130-135
Number of pages6
JournalCurrent Opinion in Biotechnology
Volume41
DOIs
Publication statusPublished - 1 Oct 2016

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