Mutation in Wilted Dwarf and Lethal 1 (WDL1) causes abnormal cuticle formation and rapid water loss in rice

Jong Jin Park, Ping Jin, Jinmi Yoon, Jung Il Yang, Hee Joong Jeong, Kosala Ranathunge, Lukas Schreiber, Rochus Franke, In Jung Lee, Gynheung An

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Epidermal cell layers play important roles in plant defenses against various environmental stresses. Here we report the identification of a cuticle membrane mutant, wilted dwarf and lethal 1 (wdl1), from a rice T-DNA insertional population. The muant is dwarf and die at seedling stage due to increased rates of water loss. Stomatal cells and pavement cells are smaller in the mutant, suggesting that WDL1 affects epidermal cell differentiation. T-DNA was inserted into a gene that encodes a protein belonging to the SGNH subfamily, within the GDSL lipase superfamily. The WDL1-sGFP signal coincided with the RFP signal driven by AtBIP-mRFP, indicating that WDL1 is an ER protein. SEM analyses showed that their leaves have a disorganized crystal wax layer. Cross-sectioning reveals loose packing of the cuticle and irregular thickness of cell wall. Detailed analyses of the epicuticular wax showed no significant changes either in the total amount and amounts of each monomer or in the levels of lipid polymers, including cutin and other covalently bound lipids, attached to the cell wall. We propose that WDL1 is involved in cutin organization, affecting depolymerizable components.

Original languageEnglish
Pages (from-to)91-103
Number of pages13
JournalPlant Molecular Biology
Volume74
Issue number1
DOIs
Publication statusPublished - 1 Jul 2010
Externally publishedYes

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