Mutation Analysis of Interleukin-5 in an Asthmatic Cohort

E. Pereira; J. Goldblatt; P. Rye; C. Sanderson; Peter Lesouef

doi:10.1002/(SICI)1098-1004(1998)11:1<51::AID-HUMU8>3.0.CO;2-O

Mutation Analysis of Interleukin-5 in an Asthmatic Cohort

E. Pereira, J. Goldblatt, P. Rye, C. Sanderson, Peter Lesouef

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Interleukin-5 (IL-5) is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. Mutation detection studies were performed on the IL-5 gene and the alpha-chain of its receptor in 30 asthmatic and 30 nonasthmatic subjects, Single strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) did not reveal any change from the reported normal sequence in all 4 exons of IL-5 as well as the promoter and 3'-untranslated regions of the gene. No SSCP variations were seen within the complete coding sequence of the IL-5 receptor alpha-chain. Mutations of the IL-5 gene coding region, its promoter and receptor are unlikely to be common causes of an inherited predisposition to asthma. (C) 1998 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	51-54
Journal	Human Mutation
Volume	11
DOIs	https://doi.org/10.1002/(SICI)1098-1004(1998)11:1<51::AID-HUMU8>3.0.CO;2-O
Publication status	Published - 1998
Externally published	Yes

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10.1002/(SICI)1098-1004(1998)11:1<51::AID-HUMU8>3.0.CO;2-O

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title = "Mutation Analysis of Interleukin-5 in an Asthmatic Cohort",

abstract = "Interleukin-5 (IL-5) is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. Mutation detection studies were performed on the IL-5 gene and the alpha-chain of its receptor in 30 asthmatic and 30 nonasthmatic subjects, Single strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) did not reveal any change from the reported normal sequence in all 4 exons of IL-5 as well as the promoter and 3'-untranslated regions of the gene. No SSCP variations were seen within the complete coding sequence of the IL-5 receptor alpha-chain. Mutations of the IL-5 gene coding region, its promoter and receptor are unlikely to be common causes of an inherited predisposition to asthma. (C) 1998 Wiley-Liss, Inc.",

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T1 - Mutation Analysis of Interleukin-5 in an Asthmatic Cohort

AU - Pereira, E.

AU - Goldblatt, J.

AU - Rye, P.

AU - Sanderson, C.

AU - Lesouef, Peter

PY - 1998

Y1 - 1998

N2 - Interleukin-5 (IL-5) is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. Mutation detection studies were performed on the IL-5 gene and the alpha-chain of its receptor in 30 asthmatic and 30 nonasthmatic subjects, Single strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) did not reveal any change from the reported normal sequence in all 4 exons of IL-5 as well as the promoter and 3'-untranslated regions of the gene. No SSCP variations were seen within the complete coding sequence of the IL-5 receptor alpha-chain. Mutations of the IL-5 gene coding region, its promoter and receptor are unlikely to be common causes of an inherited predisposition to asthma. (C) 1998 Wiley-Liss, Inc.

AB - Interleukin-5 (IL-5) is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. Mutation detection studies were performed on the IL-5 gene and the alpha-chain of its receptor in 30 asthmatic and 30 nonasthmatic subjects, Single strand conformational polymorphism (SSCP) and heteroduplex analysis (HA) did not reveal any change from the reported normal sequence in all 4 exons of IL-5 as well as the promoter and 3'-untranslated regions of the gene. No SSCP variations were seen within the complete coding sequence of the IL-5 receptor alpha-chain. Mutations of the IL-5 gene coding region, its promoter and receptor are unlikely to be common causes of an inherited predisposition to asthma. (C) 1998 Wiley-Liss, Inc.

U2 - 10.1002/(SICI)1098-1004(1998)11:1<51::AID-HUMU8>3.0.CO;2-O

DO - 10.1002/(SICI)1098-1004(1998)11:1<51::AID-HUMU8>3.0.CO;2-O

M3 - Article

VL - 11

SP - 51

EP - 54

JO - Human Mutation

JF - Human Mutation

ER -

Mutation Analysis of Interleukin-5 in an Asthmatic Cohort

Abstract

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