Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis

S. Stoppelkamp, D.M. Reid, J. Yeoh, J. Taylor, E.J. Mckenzie, G.D. Brown, S. Gordon, John Forrester, S.Y.C. Wong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

© 2015. Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.
Original languageEnglish
Pages (from-to)398-406
JournalMolecular Immunology
Volume67
Issue number2
DOIs
Publication statusPublished - 2015

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