TY - JOUR
T1 - Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis
AU - Stoppelkamp, S.
AU - Reid, D.M.
AU - Yeoh, J.
AU - Taylor, J.
AU - Mckenzie, E.J.
AU - Brown, G.D.
AU - Gordon, S.
AU - Forrester, John
AU - Wong, S.Y.C.
PY - 2015
Y1 - 2015
N2 - © 2015. Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.
AB - © 2015. Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice.
U2 - 10.1016/j.molimm.2015.07.002
DO - 10.1016/j.molimm.2015.07.002
M3 - Article
C2 - 26216045
SN - 0161-5890
VL - 67
SP - 398
EP - 406
JO - Molecular Immunology
JF - Molecular Immunology
IS - 2
ER -