Murine bone-derived mesenchymal stem cells undergo molecular changes after a single passage in culture

Anastasia M. Hughes, Vincent Kuek, Joyce Oommen, Rishi S. Kotecha, Laurence C. Cheung

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The rarity of the mesenchymal stem cell (MSC) population poses a significant challenge for MSC research. Therefore, these cells are often expanded in vitro, prior to use. However, long-term culture has been shown to alter primary MSC properties. Additionally, early passage primary MSCs in culture are often assumed to represent the primary MSC population in situ, however, little research has been done to support this. Here, we compared the transcriptomic profiles of murine MSCs freshly isolated from the bone marrow to those that had been expanded in culture for 10 days. We identified that a single passage in culture extensively altered MSC molecular signatures associated with cell cycling, differentiation and immune response. These findings indicate the critical importance of the MSC source, highlighting the need for optimization of culture conditions to minimize the impact on MSC biology and a transition towards in vivo methodologies for the study of MSC function.

Original languageEnglish
Article number12396
JournalScientific Reports
Volume14
Issue number1
DOIs
Publication statusPublished - Dec 2024

Fingerprint

Dive into the research topics of 'Murine bone-derived mesenchymal stem cells undergo molecular changes after a single passage in culture'. Together they form a unique fingerprint.

Cite this