TY - JOUR
T1 - Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia
AU - Wannigama, Dhammika Leshan
AU - Amarasiri, Mohan
AU - Hongsing, Parichart
AU - Hurst, Cameron
AU - Modchang, Charin
AU - Chadsuthi, Sudarat
AU - Anupong, Suparinthon
AU - Phattharapornjaroen, Phatthranit
AU - Rad, Ali Hosseini S. M.
AU - Fernandez, Stefan
AU - Huang, Angkana T.
AU - Kueakulpattana, Naris
AU - Tanasatitchai, Chanikan
AU - Vatanaprasan, Porames
AU - Saethang, Thammakorn
AU - Luk-in, Sirirat
AU - Storer, Robin James
AU - Ounjai, Puey
AU - Ragupathi, Naveen Kumar Devanga
AU - Kanthawee, Phitsanuruk
AU - Sano, Daisuke
AU - Furukawa, Takashi
AU - Sei, Kazunari
AU - Leelahavanichkul, Asada
AU - Kanjanabuch, Talerngsak
AU - Hirankarn, Nattiya
AU - Higgins, Paul G.
AU - Kicic, Anthony
AU - Chatsuwan, Tanittha
AU - McLellan, Alexander D.
AU - Abe, Shuichi
PY - 2023/2/1
Y1 - 2023/2/1
N2 - The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in waste-water from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.
AB - The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in waste-water from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks.
KW - Monkeypox
KW - WBE
KW - Monkeypox viral DNA
KW - Wastewater surveillance
KW - Monkeypox virus in wastewater
KW - Monkeypox Asia
UR - http://www.scopus.com/inward/record.url?scp=85143354833&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2022.159816
DO - 10.1016/j.scitotenv.2022.159816
M3 - Article
C2 - 36461562
SN - 0048-9697
VL - 858
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 159816
ER -
