Multimodal optical coherence microscopy, mechano-microscopy and fluorescence microscopy for three-dimensional characterization of multicellular spheroids

Alireza Mowla, Matt S. Hepburn, Jiayue Li, Liisa M. Hirvonen, Danielle Vahala, Sebastian Amos, Samuel Maher, Yu Suk Choi, Brendan F. Kennedy

Research output: Chapter in Book/Conference paperConference paperpeer-review


Multicellular spheroids are a powerful model to study biochemical and biophysical interactions between cancer cells during growth and progression. However, little is known about how the biomechanics of the three-dimensional (3-D) microenvironment control cancer cell behaviors due to the lack of enabling technologies that can measure 3-D subcellular-scale elasticity and co-register it with the morphology and function of cells in a 3-D microenvironment. Here, we propose a multimodal imaging system that integrates an optical coherence microscopy-based subcellular mechano-microscopy system with a multi-channel confocal fluorescence microscopy system. Using this multimodal imaging system, we scan non-metastatic MCF7 breast cancer cell spheroids encapsulated in gelatin methacryloyl (GelMA) hydrogels and co-register 3-D intra-spheroid elasticity with subcellular structures, such as nuclei and cell membranes.

Original languageEnglish
Title of host publicationOptical Coherence Imaging Techniques and Imaging in Scattering Media V
EditorsBenjamin J. Vakoc, Maciej Wojtkowski, Yoshiaki Yasuno
Place of PublicationGermany
ISBN (Electronic)9781510664739
Publication statusPublished - 11 Aug 2023
EventOptical Coherence Imaging Techniques and Imaging in Scattering Media V 2023 - Munich, Germany
Duration: 25 Jun 202329 Jun 2023

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceOptical Coherence Imaging Techniques and Imaging in Scattering Media V 2023

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