Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

Aaron B. Beasley; Emmanuel Acheampong; Weitao Lin; Elin S. Gray

doi:10.1007/978-1-0716-1205-7_16

Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

Aaron B. Beasley
, Emmanuel Acheampong
, Weitao Lin
, Elin S. Gray

Research output: Chapter in Book/Conference paper › Chapter › peer-review

5 Citations (Scopus)

Abstract

Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.

Original language	English
Title of host publication	Melanoma
Subtitle of host publication	Methods and Protocols
Editors	Kristian M. Hargadon
Publisher	Humana Press Inc.
Pages	213-222
Number of pages	10
ISBN (Electronic)	9781071612057
DOIs	https://doi.org/10.1007/978-1-0716-1205-7_16
Publication status	Published - 2021
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2265
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1007/978-1-0716-1205-7_16

Cite this

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title = "Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells",

abstract = "Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.",

keywords = "Circulating tumor cells (CTCs), Immunomagnetic beads, Multi-marker",

author = "Beasley, \{Aaron B.\} and Emmanuel Acheampong and Weitao Lin and Gray, \{Elin S.\}",

year = "2021",

doi = "10.1007/978-1-0716-1205-7\_16",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "213--222",

editor = "Hargadon, \{Kristian M.\}",

booktitle = "Melanoma",

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TY - CHAP

T1 - Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

AU - Beasley, Aaron B.

AU - Acheampong, Emmanuel

AU - Lin, Weitao

AU - Gray, Elin S.

PY - 2021

Y1 - 2021

N2 - Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.

AB - Within the last decade, circulating tumor cells (CTCs) have emerged as a promising biomarker for prognostication, treatment monitoring, and detection of markers of treatment resistance, and their isolation can be used as a minimally invasive means of profiling tumors across multiple body sites. However, CTCs represent a minuscule fraction of the total circulating cells in a patient. Therefore, sensitive isolation methods are needed for the detection and downstream analysis of these cells. Herein we describe a sensitive method for melanoma CTC isolation using a multi-marker immunomagnetic bead method. This method has been purposely optimized to detect CTCs in melanoma patients.

KW - Circulating tumor cells (CTCs)

KW - Immunomagnetic beads

KW - Multi-marker

UR - https://www.scopus.com/pages/publications/85102740706

U2 - 10.1007/978-1-0716-1205-7_16

DO - 10.1007/978-1-0716-1205-7_16

M3 - Chapter

C2 - 33704717

T3 - Methods in Molecular Biology

SP - 213

EP - 222

BT - Melanoma

A2 - Hargadon, Kristian M.

PB - Humana Press Inc.

ER -

Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells

Abstract

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