TY - JOUR
T1 - MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins
AU - Davies, Stefan
AU - Lopez Sanchez, M.I.
AU - Narsai, Reena
AU - Shearwood, Anne-Marie
AU - Mohamad Razif, M.F.
AU - Small, Ian
AU - Whelan, James
AU - Rackham, Oliver
AU - Filipovska, Aleksandra
PY - 2012/10/19
Y1 - 2012/10/19
N2 - Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNAGlu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
AB - Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNAGlu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
U2 - 10.1016/j.febslet.2012.07.043
DO - 10.1016/j.febslet.2012.07.043
M3 - Article
C2 - 22841715
VL - 586
SP - 3555
EP - 3561
JO - Federation of European Biochemical Societies Letters
JF - Federation of European Biochemical Societies Letters
SN - 0014-5793
ER -