MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

Stefan Davies; M.I. Lopez Sanchez; Reena Narsai; Anne-Marie Shearwood; M.F. Mohamad Razif; Ian Small; James Whelan; Oliver Rackham; Aleksandra Filipovska

doi:10.1016/j.febslet.2012.07.043

MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

Stefan Davies, M.I. Lopez Sanchez, Reena Narsai, Anne-Marie Shearwood, M.F. Mohamad Razif, Ian Small, James Whelan, Oliver Rackham, Aleksandra Filipovska

Research output: Contribution to journal › Article

38 Citations (Scopus)

Abstract

Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNAGlu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	3555-3561
Journal	FEBS Letters
Volume	586
DOIs	https://doi.org/10.1016/j.febslet.2012.07.043
Publication status	Published - 19 Oct 2012

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@article{4a0f0a2e4b684843b91b8a43358dd27b,

title = "MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins",

abstract = "Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNAGlu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity. Copyright {\textcopyright} 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

author = "Stefan Davies and {Lopez Sanchez}, M.I. and Reena Narsai and Anne-Marie Shearwood and {Mohamad Razif}, M.F. and Ian Small and James Whelan and Oliver Rackham and Aleksandra Filipovska",

year = "2012",

month = oct,

day = "19",

doi = "10.1016/j.febslet.2012.07.043",

language = "English",

volume = "586",

pages = "3555--3561",

journal = "Federation of European Biochemical Societies Letters",

issn = "0014-5793",

publisher = "Elsevier",

}

MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins. / Davies, Stefan; Lopez Sanchez, M.I.; Narsai, Reena; Shearwood, Anne-Marie; Mohamad Razif, M.F.; Small, Ian; Whelan, James; Rackham, Oliver ; Filipovska, Aleksandra.

In: FEBS Letters, Vol. 586, 19.10.2012, p. 3555-3561.

Research output: Contribution to journal › Article

TY - JOUR

T1 - MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins

AU - Davies, Stefan

AU - Lopez Sanchez, M.I.

AU - Narsai, Reena

AU - Shearwood, Anne-Marie

AU - Mohamad Razif, M.F.

AU - Small, Ian

AU - Whelan, James

AU - Rackham, Oliver

AU - Filipovska, Aleksandra

PY - 2012/10/19

Y1 - 2012/10/19

N2 - Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNAGlu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNAGlu, however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

U2 - 10.1016/j.febslet.2012.07.043

DO - 10.1016/j.febslet.2012.07.043

M3 - Article

C2 - 22841715

VL - 586

SP - 3555

EP - 3561

JO - Federation of European Biochemical Societies Letters

JF - Federation of European Biochemical Societies Letters

SN - 0014-5793

ER -