Morphological features of Murray Valley encephalitis virus infection in the central nervous system of swiss mice

Vance Matthews, T. Robertson, Tulene Kendrick, M. Abdo, M. Abdo, J. Papadimitriou, John Papadimitriou, P. Mcminn

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Abstract

We have examined the histological and ultrastructural features of CNS infection with Murray Valley encephalitis (MVE) virus in mice inoculated with a virulent parental strain (BH3479). Light microscopic examination revealed neuronal necrosis in the olfactory bulb and hippocampus of MVE-infected brains by 5 days post-infection (pi). Electron microscopy of these regions showed endoplasmic reticulum membrane proliferation, and tubular and spherical structures in the cisternae of the endoplasmic reticulum, Golgi complex and nuclear envelope. At seven to eight days pi, infected neurones exhibited chromatin condensation and extrusion, nuclear fragmentation, loss of segments of the nuclear envelope, reduced surface contact with adjacent cells and loss of cytoplasmic organelles. This cell injury was particularly noticeable in the proximal CA3 and distal CA1 regions of the hippocampus. The inflammatory cell profile consisted of macrophages, lymphocytes and especially neutrophils, and many of these inflammatory cells were apoptotic. High mortality rates in the BH3479-infected population of mice correlated with the intense polymorphonuclear and mononuclear leucocyte inflammatory infiltrate in the CNS.
Original languageEnglish
Pages (from-to)31-40
JournalInternational Journal of Experimental Pathology
Volume81
DOIs
Publication statusPublished - 2000

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Murray Valley encephalitis virus
Virus Diseases
Central Nervous System
Nuclear Envelope
Endoplasmic Reticulum
Hippocampus
Neutrophils
Infection
Mononuclear Leukocytes
Olfactory Bulb
Golgi Apparatus
Encephalitis
Organelles
Chromatin
Electron Microscopy
Necrosis
Macrophages
Lymphocytes
Neurons
Light

Cite this

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title = "Morphological features of Murray Valley encephalitis virus infection in the central nervous system of swiss mice",
abstract = "We have examined the histological and ultrastructural features of CNS infection with Murray Valley encephalitis (MVE) virus in mice inoculated with a virulent parental strain (BH3479). Light microscopic examination revealed neuronal necrosis in the olfactory bulb and hippocampus of MVE-infected brains by 5 days post-infection (pi). Electron microscopy of these regions showed endoplasmic reticulum membrane proliferation, and tubular and spherical structures in the cisternae of the endoplasmic reticulum, Golgi complex and nuclear envelope. At seven to eight days pi, infected neurones exhibited chromatin condensation and extrusion, nuclear fragmentation, loss of segments of the nuclear envelope, reduced surface contact with adjacent cells and loss of cytoplasmic organelles. This cell injury was particularly noticeable in the proximal CA3 and distal CA1 regions of the hippocampus. The inflammatory cell profile consisted of macrophages, lymphocytes and especially neutrophils, and many of these inflammatory cells were apoptotic. High mortality rates in the BH3479-infected population of mice correlated with the intense polymorphonuclear and mononuclear leucocyte inflammatory infiltrate in the CNS.",
author = "Vance Matthews and T. Robertson and Tulene Kendrick and M. Abdo and M. Abdo and J. Papadimitriou and John Papadimitriou and P. Mcminn",
year = "2000",
doi = "10.1046/j.1365-2613.2000.00135.x",
language = "English",
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pages = "31--40",
journal = "International Journal of Experimental Pathology",
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Morphological features of Murray Valley encephalitis virus infection in the central nervous system of swiss mice. / Matthews, Vance; Robertson, T.; Kendrick, Tulene; Abdo, M.; Abdo, M.; Papadimitriou, J.; Papadimitriou, John; Mcminn, P.

In: International Journal of Experimental Pathology, Vol. 81, 2000, p. 31-40.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Morphological features of Murray Valley encephalitis virus infection in the central nervous system of swiss mice

AU - Matthews, Vance

AU - Robertson, T.

AU - Kendrick, Tulene

AU - Abdo, M.

AU - Abdo, M.

AU - Papadimitriou, J.

AU - Papadimitriou, John

AU - Mcminn, P.

PY - 2000

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AB - We have examined the histological and ultrastructural features of CNS infection with Murray Valley encephalitis (MVE) virus in mice inoculated with a virulent parental strain (BH3479). Light microscopic examination revealed neuronal necrosis in the olfactory bulb and hippocampus of MVE-infected brains by 5 days post-infection (pi). Electron microscopy of these regions showed endoplasmic reticulum membrane proliferation, and tubular and spherical structures in the cisternae of the endoplasmic reticulum, Golgi complex and nuclear envelope. At seven to eight days pi, infected neurones exhibited chromatin condensation and extrusion, nuclear fragmentation, loss of segments of the nuclear envelope, reduced surface contact with adjacent cells and loss of cytoplasmic organelles. This cell injury was particularly noticeable in the proximal CA3 and distal CA1 regions of the hippocampus. The inflammatory cell profile consisted of macrophages, lymphocytes and especially neutrophils, and many of these inflammatory cells were apoptotic. High mortality rates in the BH3479-infected population of mice correlated with the intense polymorphonuclear and mononuclear leucocyte inflammatory infiltrate in the CNS.

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