Morphologic Observations and Novel 18S rRNA Sequences of Abbreviata hastaspicula and Abbreviata antarctica from Varanus spp. Lizards in Australia

Research output: Contribution to journalArticle

Abstract

The nematodes Abbreviata antarctica von Linstow, 1899, and Abbreviata hastaspicula Jones, 1979, are predominant spirurid nematodes in species of Varanus lizards in Australia. However, genetic knowledge of these two species of nematode is lacking. In this study, nematodes removed from Varanus gouldii were examined using integrated morphologic and molecular methods. We extracted DNA from A. hastaspicula and A. antarctica for PCR and sequencing. Specific 18S small subunit ribosomal RNA (rRNA) primers were designed on the basis of existing 18S rRNA sequences of Physalopterinae strains. Species of Abbreviata, which are closely similar morphologically, may be misidentified, especially the larvae of different species of Abbreviata that cannot be differentiated. The findings of this study will improve the accuracy in identification of A. hastaspicula and A. antarctica, both morphologically and molecularly.

Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalJournal of Wildlife Diseases
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 2019

Cite this

@article{1f09bff00433490383028d30c7056b43,
title = "Morphologic Observations and Novel 18S rRNA Sequences of Abbreviata hastaspicula and Abbreviata antarctica from Varanus spp. Lizards in Australia",
abstract = "The nematodes Abbreviata antarctica von Linstow, 1899, and Abbreviata hastaspicula Jones, 1979, are predominant spirurid nematodes in species of Varanus lizards in Australia. However, genetic knowledge of these two species of nematode is lacking. In this study, nematodes removed from Varanus gouldii were examined using integrated morphologic and molecular methods. We extracted DNA from A. hastaspicula and A. antarctica for PCR and sequencing. Specific 18S small subunit ribosomal RNA (rRNA) primers were designed on the basis of existing 18S rRNA sequences of Physalopterinae strains. Species of Abbreviata, which are closely similar morphologically, may be misidentified, especially the larvae of different species of Abbreviata that cannot be differentiated. The findings of this study will improve the accuracy in identification of A. hastaspicula and A. antarctica, both morphologically and molecularly.",
keywords = "Abbreviata spp., morphology, Physalopterinae, Spirurina nematodes, SSU rRNA, small subunit, 18S rRNA, PHYSALOPTERIDAE, NEMATODES, PHYLOGENY",
author = "Chloe King and Tay, {Chin Y.} and Jones, {Hugh I.}",
year = "2019",
month = "1",
doi = "10.7589/2017-11-272",
language = "English",
volume = "55",
pages = "149--152",
journal = "Journal of Wildlife Diseases",
issn = "0090-3558",
publisher = "WILDLIFE DISEASE ASSOC, INC",
number = "1",

}

TY - JOUR

T1 - Morphologic Observations and Novel 18S rRNA Sequences of Abbreviata hastaspicula and Abbreviata antarctica from Varanus spp. Lizards in Australia

AU - King, Chloe

AU - Tay, Chin Y.

AU - Jones, Hugh I.

PY - 2019/1

Y1 - 2019/1

N2 - The nematodes Abbreviata antarctica von Linstow, 1899, and Abbreviata hastaspicula Jones, 1979, are predominant spirurid nematodes in species of Varanus lizards in Australia. However, genetic knowledge of these two species of nematode is lacking. In this study, nematodes removed from Varanus gouldii were examined using integrated morphologic and molecular methods. We extracted DNA from A. hastaspicula and A. antarctica for PCR and sequencing. Specific 18S small subunit ribosomal RNA (rRNA) primers were designed on the basis of existing 18S rRNA sequences of Physalopterinae strains. Species of Abbreviata, which are closely similar morphologically, may be misidentified, especially the larvae of different species of Abbreviata that cannot be differentiated. The findings of this study will improve the accuracy in identification of A. hastaspicula and A. antarctica, both morphologically and molecularly.

AB - The nematodes Abbreviata antarctica von Linstow, 1899, and Abbreviata hastaspicula Jones, 1979, are predominant spirurid nematodes in species of Varanus lizards in Australia. However, genetic knowledge of these two species of nematode is lacking. In this study, nematodes removed from Varanus gouldii were examined using integrated morphologic and molecular methods. We extracted DNA from A. hastaspicula and A. antarctica for PCR and sequencing. Specific 18S small subunit ribosomal RNA (rRNA) primers were designed on the basis of existing 18S rRNA sequences of Physalopterinae strains. Species of Abbreviata, which are closely similar morphologically, may be misidentified, especially the larvae of different species of Abbreviata that cannot be differentiated. The findings of this study will improve the accuracy in identification of A. hastaspicula and A. antarctica, both morphologically and molecularly.

KW - Abbreviata spp.

KW - morphology

KW - Physalopterinae

KW - Spirurina nematodes

KW - SSU rRNA

KW - small subunit

KW - 18S rRNA

KW - PHYSALOPTERIDAE

KW - NEMATODES

KW - PHYLOGENY

U2 - 10.7589/2017-11-272

DO - 10.7589/2017-11-272

M3 - Article

VL - 55

SP - 149

EP - 152

JO - Journal of Wildlife Diseases

JF - Journal of Wildlife Diseases

SN - 0090-3558

IS - 1

ER -