Mononuclear Cell Factors that Inhibit Fibroblast Collagen Synthesis: II. Properties of the Factors

P. H. HART, L. W. POWELL, W. G.E. COOKSLEY, J. W. HALLIDAY

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Supernatants harvested from peripheral blood mononuclear cells (PBMC) incubated either with the non‐specific mitogen phytohaemagglutinin‐P (PHA) or with the specific antigen tuberculin purified protein derivative for 72 h decreased collagen synthesis by dermal fibroblasts. PHA‐induced mononuclear cell factors (PHA‐MCF) responsible for decreases in collagen synthesis by dermal fibroblasts were localized by column chromatography on Sephacryl S‐200 to fractions of 30,000‐60,000 daltons. Proteolytic enzymes destroyed the activity of PHA‐MCF, but after incubation with neuraminidase some activity of these factors remained, The activity of PHA‐MCF was not inhibited by incubation with the monosaccharides L‐fucose, L‐rhamnose. N‐acetylglucosamine, and α‐melhyl‐D‐mannoside but was partially destroyed by heating at 80°C for 10 min. The factors were not mitogenic to PBMC. These factors did not appear to resemble any previously characterized factors produced by non‐adherent mononuclear cells. The mechanism by which these factors decreased fibroblast collagen synthesis appeared complex. There was no detectable increase in the release of collagenasc by fibroblasts, nor was a cytotoxic effect apparent. Increased PGE2 production by fibroblasts could not be related to the factor‐induced decrease in fibroblast collagen synthesis.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalScandinavian Journal of Immunology
Volume18
Issue number1
DOIs
Publication statusPublished - 1 Jan 1983
Externally publishedYes

Fingerprint Dive into the research topics of 'Mononuclear Cell Factors that Inhibit Fibroblast Collagen Synthesis: II. Properties of the Factors'. Together they form a unique fingerprint.

  • Cite this