Monitoring interactions between G-protein-coupled receptors and ß-arrestins

Kevin Pfleger, Matthew Dalrymple, Jasmin Dromey, Karin Eidne

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

ß-Arrestins 1 and 2 are ubiquitously expressed intracellular adaptor and scaffolding proteins that play important roles in GPCR (G-protein-coupled receptor) desensitization, internalization, intracellular trafficking and G-protein-independent signalling. Recent developments in BRET (bioluminescence resonance energy transfer) technology enable novel insights to be gained from real-time monitoring of GPCR-ss-arrestin complexes in live cells for prolonged periods. in concert with confocal microscopy, assays for studying internalization and recycling kinetics such as ELISAs, and techniques for measuring downstream signalling pathways such as those involving MAPKs (mitogen-activated protein kinases), investigators can now use a range of experimental tools to elucidate the ever-expanding roles of ss-arrestins in mediating GPCR function.
Original languageEnglish
Pages (from-to)764-766
JournalBiochemical Society Transactions
Volume35
Issue number4
Publication statusPublished - 2007

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