Molecular techniques and their limitations shape our view of the holobiont

Consortium Australian Acad Sci Bod

Research output: Contribution to journalReview article

Abstract

It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

Original languageEnglish
Article number125695
Number of pages8
JournalZoology
Volume137
DOIs
Publication statusPublished - Dec 2019

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