TY - JOUR
T1 - Molecular Markers for Identifying Resistance Genes in Brassica napus
AU - Van de Wouw, Angela P.
AU - Zhang, Yueqi
AU - Saad, Nur Shuhadah Mohd
AU - Yang, Hua
AU - Sheedy, Elizabeth
AU - Elliott, Candace E.
AU - Batley, Jacqueline
N1 - Funding Information:
Acknowledgments: The authors would like to thank the Australian breeding companies for access to commercial cultivars and advanced breeding lines for genotypic and phenotypic screening. We thank the Grains Research and Development Corporation for funding.
Funding Information:
This research was funded by the Grains Research and Development Corporation (UWA1905-006RTX).
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/5
Y1 - 2022/5
N2 - Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is the most devas-tating disease of canola (Brassica napus, oilseed rape) worldwide. Breeding for genetic resistance is the most widely used tool for controlling this disease and minimizing the impact on yield. To date, five resistance genes (Rlm2, LepR3, Rlm4, Rlm7, Rlm9) have been cloned from B. napus, representing alleles of two different gene loci, Rlm2-LepR3 and Rlm4-7-9. We report on the development and validation of Kompetitive Allele-Specific PCR (KASP) markers that can discriminate between the resistant and susceptible alleles of each resistance gene. These markers will provide valuable tools for both researchers and industry through the ability to characterize resistance genes without phenotyping.
AB - Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is the most devas-tating disease of canola (Brassica napus, oilseed rape) worldwide. Breeding for genetic resistance is the most widely used tool for controlling this disease and minimizing the impact on yield. To date, five resistance genes (Rlm2, LepR3, Rlm4, Rlm7, Rlm9) have been cloned from B. napus, representing alleles of two different gene loci, Rlm2-LepR3 and Rlm4-7-9. We report on the development and validation of Kompetitive Allele-Specific PCR (KASP) markers that can discriminate between the resistant and susceptible alleles of each resistance gene. These markers will provide valuable tools for both researchers and industry through the ability to characterize resistance genes without phenotyping.
KW - blackleg
KW - canola
KW - Kompetitive Allele-Specific PCR (KASP)
KW - Leptosphaeria maculans
KW - molecular breeding
UR - http://www.scopus.com/inward/record.url?scp=85129523413&partnerID=8YFLogxK
U2 - 10.3390/agronomy12050985
DO - 10.3390/agronomy12050985
M3 - Article
AN - SCOPUS:85129523413
SN - 2073-4395
VL - 12
JO - Agronomy
JF - Agronomy
IS - 5
M1 - 985
ER -