Molecular investigation of Notch signalling on transcriptional regulation of the human Complement Receptor 2 (CD21/CR2) gene

Hans Ng

Research output: ThesisDoctoral Thesis

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Abstract

Complement receptor 2 (CR2) is a cell surface marker on B cells. Expression of CR2 is tightly regulated during B lymphopoiesis. We investigated the role of Notch signalling in regulating CR2 expression by utilising a Notch co-culture system. Following Notch activation in pre-B and mature B cells, increased CR2 mRNA was observed. Chromatin accessibility assays revealed that Notch signalling poised the CR2 gene for transcription. Functional analysis demonstrated recruitment of Notch­associated transcription factors to its cognate site within the CR2 promoter. In summary, we revealed the complexity of Notch signalling in regulating CR2 expression in pre-B and mature B cells.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Quail, Elizabeth, Supervisor
  • Ulgiati, Daniela, Supervisor
Thesis sponsors
Award date8 Aug 2019
DOIs
Publication statusUnpublished - 2019

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