Molecular investigation of Notch signalling on transcriptional regulation of the human Complement Receptor 2 (CD21/CR2) gene

Hans Ng

doi:10.26182/5d672ae057acb

Molecular investigation of Notch signalling on transcriptional regulation of the human Complement Receptor 2 (CD21/CR2) gene

Hans Ng

School of Biomedical Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

Complement receptor 2 (CR2) is a cell surface marker on B cells. Expression of CR2 is tightly regulated during B lymphopoiesis. We investigated the role of Notch signalling in regulating CR2 expression by utilising a Notch co-culture system. Following Notch activation in pre-B and mature B cells, increased CR2 mRNA was observed. Chromatin accessibility assays revealed that Notch signalling poised the CR2 gene for transcription. Functional analysis demonstrated recruitment of Notchassociated transcription factors to its cognate site within the CR2 promoter. In summary, we revealed the complexity of Notch signalling in regulating CR2 expression in pre-B and mature B cells.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Quail, Elizabeth, Supervisor Ulgiati, Daniela, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	8 Aug 2019
DOIs	https://doi.org/10.26182/5d672ae057acb
Publication status	Unpublished - 2019

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10.26182/5d672ae057acb

THESIS - DOCTOR OF PHILOSOPHY - NG Han Leng - 2019
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title = "Molecular investigation of Notch signalling on transcriptional regulation of the human Complement Receptor 2 (CD21/CR2) gene",

abstract = "Complement receptor 2 (CR2) is a cell surface marker on B cells. Expression of CR2 is tightly regulated during B lymphopoiesis. We investigated the role of Notch signalling in regulating CR2 expression by utilising a Notch co-culture system. Following Notch activation in pre-B and mature B cells, increased CR2 mRNA was observed. Chromatin accessibility assays revealed that Notch signalling poised the CR2 gene for transcription. Functional analysis demonstrated recruitment of Notchassociated transcription factors to its cognate site within the CR2 promoter. In summary, we revealed the complexity of Notch signalling in regulating CR2 expression in pre-B and mature B cells. ",

keywords = "Complement Receptor 2, Gene regulation, Notch signalling, Transcription factors, B lymphocytes, lmmunology, B lymphopoiesis, Chromatin accessibility",

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year = "2019",

doi = "10.26182/5d672ae057acb",

language = "English",

school = "The University of Western Australia",

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T1 - Molecular investigation of Notch signalling on transcriptional regulation of the human Complement Receptor 2 (CD21/CR2) gene

AU - Ng, Hans

PY - 2019

Y1 - 2019

N2 - Complement receptor 2 (CR2) is a cell surface marker on B cells. Expression of CR2 is tightly regulated during B lymphopoiesis. We investigated the role of Notch signalling in regulating CR2 expression by utilising a Notch co-culture system. Following Notch activation in pre-B and mature B cells, increased CR2 mRNA was observed. Chromatin accessibility assays revealed that Notch signalling poised the CR2 gene for transcription. Functional analysis demonstrated recruitment of Notchassociated transcription factors to its cognate site within the CR2 promoter. In summary, we revealed the complexity of Notch signalling in regulating CR2 expression in pre-B and mature B cells.

AB - Complement receptor 2 (CR2) is a cell surface marker on B cells. Expression of CR2 is tightly regulated during B lymphopoiesis. We investigated the role of Notch signalling in regulating CR2 expression by utilising a Notch co-culture system. Following Notch activation in pre-B and mature B cells, increased CR2 mRNA was observed. Chromatin accessibility assays revealed that Notch signalling poised the CR2 gene for transcription. Functional analysis demonstrated recruitment of Notchassociated transcription factors to its cognate site within the CR2 promoter. In summary, we revealed the complexity of Notch signalling in regulating CR2 expression in pre-B and mature B cells.

KW - Complement Receptor 2

KW - Gene regulation

KW - Notch signalling

KW - Transcription factors

KW - B lymphocytes

KW - lmmunology

KW - B lymphopoiesis

KW - Chromatin accessibility

U2 - 10.26182/5d672ae057acb

DO - 10.26182/5d672ae057acb

M3 - Doctoral Thesis

ER -

Molecular investigation of Notch signalling on transcriptional regulation of the human Complement Receptor 2 (CD21/CR2) gene

Abstract

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