Abstract
[Truncated] Cauliflower production in Australia is export oriented. The industry aims for uniform, high quality product and is based on F1 hybrid cauliflower cultivars. However non-uniform products still occur. The lack of uniformity may be due to genetic variation evident in the seed, seedling or production stage.
There are many cauliflower cultivars released each year and there is a need to correctly identify cultivars for seed companies and growers. We developed fingerprinting keys using molecular markers to correctly identify cultivars at any stage, and therefore without the need for field trials. Australian F1 hybrid cultivars and open pollinated cultivars of Indonesia were assessed genetically using RAPD markers. Genetic variation within and between cultivars of both countries were investigated. Dendograms were constructed using Neighbor-Joining analysis based on Phylogenetic Analysis Using Parsimony (PAUP). DNA fingerprinting keys were developed and genetic relationships among cultivars were determined. Comparison between Indonesian and Australian based cultivars indicated that Indonesian cultivars have unique genotypes and would be good sources of genes for future crop improvement. Results proved that RAPD markers can be used for the routine identification of cauliflower cultivars.
There are many cauliflower cultivars released each year and there is a need to correctly identify cultivars for seed companies and growers. We developed fingerprinting keys using molecular markers to correctly identify cultivars at any stage, and therefore without the need for field trials. Australian F1 hybrid cultivars and open pollinated cultivars of Indonesia were assessed genetically using RAPD markers. Genetic variation within and between cultivars of both countries were investigated. Dendograms were constructed using Neighbor-Joining analysis based on Phylogenetic Analysis Using Parsimony (PAUP). DNA fingerprinting keys were developed and genetic relationships among cultivars were determined. Comparison between Indonesian and Australian based cultivars indicated that Indonesian cultivars have unique genotypes and would be good sources of genes for future crop improvement. Results proved that RAPD markers can be used for the routine identification of cauliflower cultivars.
| Original language | English |
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| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| DOIs | |
| Publication status | Unpublished - 2006 |
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