Molecular imaging needles: Dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue

Loretta Scolaro, Dirk Lorenser, W.J. Madore, Rodney Kirk, Anne Kramer, George Yeoh, N. Godbout, David Sampson, C. Boudoux, Robert Mclaughlin

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

© 2015 Optical Society of America. Molecular imaging using optical techniques provides insight into disease at the cellular level. In this paper, we report on a novel dualmodality probe capable of performing molecular imaging by combining simultaneous three-dimensional optical coherence tomography (OCT) and two-dimensional fluorescence imaging in a hypodermic needle. The probe, referred to as a molecular imaging (MI) needle, may be inserted tens of millimeters into tissue. The MI needle utilizes double-clad fiber to carry both imaging modalities, and is interfaced to a 1310-nm OCT system and a fluorescence imaging subsystem using an asymmetrical double-clad fiber coupler customized to achieve high fluorescence collection efficiency. We present, to the best of our knowledge, the first dual-modality OCT and fluorescence needle probe with sufficient sensitivity to image fluorescently labeled antibodies. Such probes enable high-resolution molecular imaging deep within tissue.
Original languageEnglish
Pages (from-to)1767-1781
JournalBiomedical Optics Express
Volume6
Issue number5
DOIs
Publication statusPublished - 2015

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