Molecular Classification of Autofluorescence Excision Margins in Oral Potentially Malignant Disorders

Camile S. Farah, Farzaneh Kordbacheh, Keziah John, Nigel Bennett, Simon A. Fox

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE: To define molecular differences between autofluorescence and white light defined excision margins in oral potentially malignant disorders (OPMD) using transcriptome expression profiles.

MATERIALS AND METHODS: Excisional biopsy specimens were taken from 11 patients at three different sites for each lesion: centre, white light margin, and autofluorescence margin. The lesions were diagnosed histopathologically as oral epithelial dysplasia, oral lichenoid dysplasia, oral lichen planus or other. Transcriptome analysis was performed by RNA sequencing, hierarchical clustering, differential expression and biological pathway analysis.

RESULTS: For hierarchical clustering the samples broadly clustered according to histology rather than the margins with lichenoid samples clustering together. Differential expression analysis showed that independent of histology, there was greater molecular dysregulation between the lesion centre and autofluorescence margin compared to the lesion centre and white light margin. Furthermore, the autofluorescence and white light margins were molecularly distinct indicating the white light margins harboured abnormality.

CONCLUSION: Our results indicate that the molecular profile of OPMD changes with divergence away from the centre of the lesion, and that autofluorescence determined margins are superior to the white light margin in achieving a clear molecular margin when excising an OPMD. This article is protected by copyright. All rights reserved.

Original languageEnglish
JournalOral Diseases
DOIs
Publication statusE-pub ahead of print - 14 Dec 2017

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Light
Cluster Analysis
Histology
Oral Lichen Planus
RNA Sequence Analysis
Gene Expression Profiling
Transcriptome
Margins of Excision
Biopsy

Cite this

Farah, Camile S. ; Kordbacheh, Farzaneh ; John, Keziah ; Bennett, Nigel ; Fox, Simon A. / Molecular Classification of Autofluorescence Excision Margins in Oral Potentially Malignant Disorders. In: Oral Diseases. 2017.
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abstract = "OBJECTIVE: To define molecular differences between autofluorescence and white light defined excision margins in oral potentially malignant disorders (OPMD) using transcriptome expression profiles.MATERIALS AND METHODS: Excisional biopsy specimens were taken from 11 patients at three different sites for each lesion: centre, white light margin, and autofluorescence margin. The lesions were diagnosed histopathologically as oral epithelial dysplasia, oral lichenoid dysplasia, oral lichen planus or other. Transcriptome analysis was performed by RNA sequencing, hierarchical clustering, differential expression and biological pathway analysis.RESULTS: For hierarchical clustering the samples broadly clustered according to histology rather than the margins with lichenoid samples clustering together. Differential expression analysis showed that independent of histology, there was greater molecular dysregulation between the lesion centre and autofluorescence margin compared to the lesion centre and white light margin. Furthermore, the autofluorescence and white light margins were molecularly distinct indicating the white light margins harboured abnormality.CONCLUSION: Our results indicate that the molecular profile of OPMD changes with divergence away from the centre of the lesion, and that autofluorescence determined margins are superior to the white light margin in achieving a clear molecular margin when excising an OPMD. This article is protected by copyright. All rights reserved.",
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Molecular Classification of Autofluorescence Excision Margins in Oral Potentially Malignant Disorders. / Farah, Camile S.; Kordbacheh, Farzaneh; John, Keziah; Bennett, Nigel; Fox, Simon A.

In: Oral Diseases, 14.12.2017.

Research output: Contribution to journalArticle

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T1 - Molecular Classification of Autofluorescence Excision Margins in Oral Potentially Malignant Disorders

AU - Farah, Camile S.

AU - Kordbacheh, Farzaneh

AU - John, Keziah

AU - Bennett, Nigel

AU - Fox, Simon A.

N1 - This article is protected by copyright. All rights reserved.

PY - 2017/12/14

Y1 - 2017/12/14

N2 - OBJECTIVE: To define molecular differences between autofluorescence and white light defined excision margins in oral potentially malignant disorders (OPMD) using transcriptome expression profiles.MATERIALS AND METHODS: Excisional biopsy specimens were taken from 11 patients at three different sites for each lesion: centre, white light margin, and autofluorescence margin. The lesions were diagnosed histopathologically as oral epithelial dysplasia, oral lichenoid dysplasia, oral lichen planus or other. Transcriptome analysis was performed by RNA sequencing, hierarchical clustering, differential expression and biological pathway analysis.RESULTS: For hierarchical clustering the samples broadly clustered according to histology rather than the margins with lichenoid samples clustering together. Differential expression analysis showed that independent of histology, there was greater molecular dysregulation between the lesion centre and autofluorescence margin compared to the lesion centre and white light margin. Furthermore, the autofluorescence and white light margins were molecularly distinct indicating the white light margins harboured abnormality.CONCLUSION: Our results indicate that the molecular profile of OPMD changes with divergence away from the centre of the lesion, and that autofluorescence determined margins are superior to the white light margin in achieving a clear molecular margin when excising an OPMD. This article is protected by copyright. All rights reserved.

AB - OBJECTIVE: To define molecular differences between autofluorescence and white light defined excision margins in oral potentially malignant disorders (OPMD) using transcriptome expression profiles.MATERIALS AND METHODS: Excisional biopsy specimens were taken from 11 patients at three different sites for each lesion: centre, white light margin, and autofluorescence margin. The lesions were diagnosed histopathologically as oral epithelial dysplasia, oral lichenoid dysplasia, oral lichen planus or other. Transcriptome analysis was performed by RNA sequencing, hierarchical clustering, differential expression and biological pathway analysis.RESULTS: For hierarchical clustering the samples broadly clustered according to histology rather than the margins with lichenoid samples clustering together. Differential expression analysis showed that independent of histology, there was greater molecular dysregulation between the lesion centre and autofluorescence margin compared to the lesion centre and white light margin. Furthermore, the autofluorescence and white light margins were molecularly distinct indicating the white light margins harboured abnormality.CONCLUSION: Our results indicate that the molecular profile of OPMD changes with divergence away from the centre of the lesion, and that autofluorescence determined margins are superior to the white light margin in achieving a clear molecular margin when excising an OPMD. This article is protected by copyright. All rights reserved.

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