Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis

U. M. Morgan; C. C. Constantine; P. O'Donoghue; B. P. Meloni; P. A. O'Brien; R. C.A. Thompson

doi:10.4269/ajtmh.1995.52.559

Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis

U. M. Morgan, C. C. Constantine, P. O'Donoghue, B. P. Meloni, P. A. O'Brien, R. C.A. Thompson

Research output: Contribution to journal › Article › peer-review

120 Citations (Scopus)

Abstract

Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating bacterial DNA. The results generated by four of the five primers were statistically correlated (P < 0.001). The combined data from three primers were used to construct a phenogram using Jaccard's distance. Four groupings could be distinguished. Two C. serpentis isolates from snakes formed a distinct group of their own, whereas C. parvum isolates were divided into two main groups: one containing most human isolates and the other containing mostly domestic animals plus two remaining human isolates. Due to the sensitivity of the RAPD technique, isolates can now be analyzed genetically, directly from fecal samples without further biological amplification. This represents a significant advance on current techniques.

Original language	English
Pages (from-to)	559-564
Number of pages	6
Journal	American Journal of Tropical Medicine and Hygiene
Volume	52
Issue number	6
DOIs	https://doi.org/10.4269/ajtmh.1995.52.559
Publication status	Published - Jun 1995
Externally published	Yes

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title = "Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis",

abstract = "Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating bacterial DNA. The results generated by four of the five primers were statistically correlated (P < 0.001). The combined data from three primers were used to construct a phenogram using Jaccard's distance. Four groupings could be distinguished. Two C. serpentis isolates from snakes formed a distinct group of their own, whereas C. parvum isolates were divided into two main groups: one containing most human isolates and the other containing mostly domestic animals plus two remaining human isolates. Due to the sensitivity of the RAPD technique, isolates can now be analyzed genetically, directly from fecal samples without further biological amplification. This represents a significant advance on current techniques.",

author = "Morgan, {U. M.} and Constantine, {C. C.} and P. O'Donoghue and Meloni, {B. P.} and O'Brien, {P. A.} and Thompson, {R. C.A.}",

year = "1995",

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doi = "10.4269/ajtmh.1995.52.559",

language = "English",

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journal = "American Journal of Tropical Medicine and Hygiene",

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TY - JOUR

T1 - Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis

AU - Morgan, U. M.

AU - Constantine, C. C.

AU - O'Donoghue, P.

AU - Meloni, B. P.

AU - O'Brien, P. A.

AU - Thompson, R. C.A.

PY - 1995/6

Y1 - 1995/6

N2 - Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating bacterial DNA. The results generated by four of the five primers were statistically correlated (P < 0.001). The combined data from three primers were used to construct a phenogram using Jaccard's distance. Four groupings could be distinguished. Two C. serpentis isolates from snakes formed a distinct group of their own, whereas C. parvum isolates were divided into two main groups: one containing most human isolates and the other containing mostly domestic animals plus two remaining human isolates. Due to the sensitivity of the RAPD technique, isolates can now be analyzed genetically, directly from fecal samples without further biological amplification. This represents a significant advance on current techniques.

AB - Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating bacterial DNA. The results generated by four of the five primers were statistically correlated (P < 0.001). The combined data from three primers were used to construct a phenogram using Jaccard's distance. Four groupings could be distinguished. Two C. serpentis isolates from snakes formed a distinct group of their own, whereas C. parvum isolates were divided into two main groups: one containing most human isolates and the other containing mostly domestic animals plus two remaining human isolates. Due to the sensitivity of the RAPD technique, isolates can now be analyzed genetically, directly from fecal samples without further biological amplification. This represents a significant advance on current techniques.

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JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 6

ER -

Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis

Abstract

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