Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat

Zitong Yu, Yanchun Peng, Md Shahidul Islam, Maoyun She, Meiqin Lu, Domenico Lafiandra, Nandita Roy, Angela Juhasz, Guijun Yan, Wujun Ma

Research output: Contribution to journalArticle

Abstract

An increased number of high molecular weight glutenin subunits (HMW-GSs) is advantageous to the formation of glutenin macropolymer, the major determinant of end-product quality. Out of the six HMW-GS genes, Glu-1Ay is typically inactive in most hexaploid wheat cultivars. In the current study, we characterized two active Glu-1Ay genes Glu-1Ay N11 and Glu-1Ay C422 from a hexaploid wheat cultivar N11 and a durum wheat cultivar C422, respectively. The molecular masses of the 1Ay subunit of N11 (Glu-1Ay N11 ) and C422 (Glu-1Ay C422 ) were 61.1 kDa and 63.9 kDa, respectively, measured by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Molecular characterization of Glu-1Ay N11 and Glu-1Ay C422 showed their complete coding regions were 1764 bp and 1827 bp in length, respectively. The deduced amino acid sequence of both Glu-1Ay N11 and Glu-1Ay C422 has a similar typical primary structure as the other y-type HMW-GSs, but in comparison to Glu-1By and Glu-1Dy subunits, the Glu-1Ay subunit has one less conserved cysteine residue in the repetitive domain. Phylogenetic analysis revealed that the inactive Glu-1Ay had a closer evolutionary relationship with Glu-1Ay C422 than with Glu-1Ay N11 . The divergence time among Glu-1Ay N11 , Glu-1Ay C422 , and other y-type subunit genes was also estimated.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalJournal of Cereal Science
Volume86
DOIs
Publication statusPublished - 1 Mar 2019

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glutenins
Triticum
Genes
Molecular Weight
Molecular weight
molecular weight
loci
wheat
phylogeny
hexaploidy
cultivars
genes
matrix-assisted laser desorption-ionization mass spectrometry
Molecular mass
durum wheat
product quality
Ionization
Mass spectrometry
Cysteine
cysteine

Cite this

Yu, Zitong ; Peng, Yanchun ; Islam, Md Shahidul ; She, Maoyun ; Lu, Meiqin ; Lafiandra, Domenico ; Roy, Nandita ; Juhasz, Angela ; Yan, Guijun ; Ma, Wujun. / Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat. In: Journal of Cereal Science. 2019 ; Vol. 86. pp. 9-14.
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abstract = "An increased number of high molecular weight glutenin subunits (HMW-GSs) is advantageous to the formation of glutenin macropolymer, the major determinant of end-product quality. Out of the six HMW-GS genes, Glu-1Ay is typically inactive in most hexaploid wheat cultivars. In the current study, we characterized two active Glu-1Ay genes Glu-1Ay N11 and Glu-1Ay C422 from a hexaploid wheat cultivar N11 and a durum wheat cultivar C422, respectively. The molecular masses of the 1Ay subunit of N11 (Glu-1Ay N11 ) and C422 (Glu-1Ay C422 ) were 61.1 kDa and 63.9 kDa, respectively, measured by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Molecular characterization of Glu-1Ay N11 and Glu-1Ay C422 showed their complete coding regions were 1764 bp and 1827 bp in length, respectively. The deduced amino acid sequence of both Glu-1Ay N11 and Glu-1Ay C422 has a similar typical primary structure as the other y-type HMW-GSs, but in comparison to Glu-1By and Glu-1Dy subunits, the Glu-1Ay subunit has one less conserved cysteine residue in the repetitive domain. Phylogenetic analysis revealed that the inactive Glu-1Ay had a closer evolutionary relationship with Glu-1Ay C422 than with Glu-1Ay N11 . The divergence time among Glu-1Ay N11 , Glu-1Ay C422 , and other y-type subunit genes was also estimated.",
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Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat. / Yu, Zitong; Peng, Yanchun; Islam, Md Shahidul; She, Maoyun; Lu, Meiqin; Lafiandra, Domenico; Roy, Nandita; Juhasz, Angela; Yan, Guijun; Ma, Wujun.

In: Journal of Cereal Science, Vol. 86, 01.03.2019, p. 9-14.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Molecular characterization and phylogenetic analysis of active y-type high molecular weight glutenin subunit genes at Glu-A1 locus in wheat

AU - Yu, Zitong

AU - Peng, Yanchun

AU - Islam, Md Shahidul

AU - She, Maoyun

AU - Lu, Meiqin

AU - Lafiandra, Domenico

AU - Roy, Nandita

AU - Juhasz, Angela

AU - Yan, Guijun

AU - Ma, Wujun

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N2 - An increased number of high molecular weight glutenin subunits (HMW-GSs) is advantageous to the formation of glutenin macropolymer, the major determinant of end-product quality. Out of the six HMW-GS genes, Glu-1Ay is typically inactive in most hexaploid wheat cultivars. In the current study, we characterized two active Glu-1Ay genes Glu-1Ay N11 and Glu-1Ay C422 from a hexaploid wheat cultivar N11 and a durum wheat cultivar C422, respectively. The molecular masses of the 1Ay subunit of N11 (Glu-1Ay N11 ) and C422 (Glu-1Ay C422 ) were 61.1 kDa and 63.9 kDa, respectively, measured by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Molecular characterization of Glu-1Ay N11 and Glu-1Ay C422 showed their complete coding regions were 1764 bp and 1827 bp in length, respectively. The deduced amino acid sequence of both Glu-1Ay N11 and Glu-1Ay C422 has a similar typical primary structure as the other y-type HMW-GSs, but in comparison to Glu-1By and Glu-1Dy subunits, the Glu-1Ay subunit has one less conserved cysteine residue in the repetitive domain. Phylogenetic analysis revealed that the inactive Glu-1Ay had a closer evolutionary relationship with Glu-1Ay C422 than with Glu-1Ay N11 . The divergence time among Glu-1Ay N11 , Glu-1Ay C422 , and other y-type subunit genes was also estimated.

AB - An increased number of high molecular weight glutenin subunits (HMW-GSs) is advantageous to the formation of glutenin macropolymer, the major determinant of end-product quality. Out of the six HMW-GS genes, Glu-1Ay is typically inactive in most hexaploid wheat cultivars. In the current study, we characterized two active Glu-1Ay genes Glu-1Ay N11 and Glu-1Ay C422 from a hexaploid wheat cultivar N11 and a durum wheat cultivar C422, respectively. The molecular masses of the 1Ay subunit of N11 (Glu-1Ay N11 ) and C422 (Glu-1Ay C422 ) were 61.1 kDa and 63.9 kDa, respectively, measured by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Molecular characterization of Glu-1Ay N11 and Glu-1Ay C422 showed their complete coding regions were 1764 bp and 1827 bp in length, respectively. The deduced amino acid sequence of both Glu-1Ay N11 and Glu-1Ay C422 has a similar typical primary structure as the other y-type HMW-GSs, but in comparison to Glu-1By and Glu-1Dy subunits, the Glu-1Ay subunit has one less conserved cysteine residue in the repetitive domain. Phylogenetic analysis revealed that the inactive Glu-1Ay had a closer evolutionary relationship with Glu-1Ay C422 than with Glu-1Ay N11 . The divergence time among Glu-1Ay N11 , Glu-1Ay C422 , and other y-type subunit genes was also estimated.

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