TY - JOUR
T1 - Mixed-Ploidy and Dysploidy in Hypericum perforatum
T2 - A Karyomorphological and Genome Size Study
AU - Mehravi, Shaghayegh
AU - Karimzadeh, Ghasem
AU - Kordenaeej, Alaeddin
AU - Hanifei, Mehrdad
N1 - Funding Information:
This research was supported by the Tarbiat Modares University, Tehran province, Iran. (Grant Number: D80/2156).
Publisher Copyright:
© 2022 by the authors.
PY - 2022/11
Y1 - 2022/11
N2 - Karyomorphology and genome size of 15 St John’s wort (Hypericum perforatum L.) populations are reported for the first time. Root tips and fresh young leaves were used for karyological studies and flow cytometric (FCM) measurements, respectively. The chromosome length varied from 0.81 µm to 1.16 µm, and chromosome types were determined as “m”. Eight different somatic chromosome numbers were found (2n = 16, 22, 24, 26, 28, 30, 32, 38). Based on the observed basic (x) chromosome numbers of x = 8, 11, 13, 14, 15, 19, this may correspond to diploid (2x), triploid (3x), tetraploid (4x), respectively. Interestingly, we found mixoploidy (3x − 4x) in the root tips of one of the populations. Hybridization, polyploidy and dysploid variation may be the main factors associated with the chromosome number evolution of this species. FCM showed that 2C DNA contents vary from 0.87 to 2.02 pg, showing more than a 2-fold variation. The mean amount of 2C DNA/chromosome and the mean of monoploid genome size were not proportional to ploidy.
AB - Karyomorphology and genome size of 15 St John’s wort (Hypericum perforatum L.) populations are reported for the first time. Root tips and fresh young leaves were used for karyological studies and flow cytometric (FCM) measurements, respectively. The chromosome length varied from 0.81 µm to 1.16 µm, and chromosome types were determined as “m”. Eight different somatic chromosome numbers were found (2n = 16, 22, 24, 26, 28, 30, 32, 38). Based on the observed basic (x) chromosome numbers of x = 8, 11, 13, 14, 15, 19, this may correspond to diploid (2x), triploid (3x), tetraploid (4x), respectively. Interestingly, we found mixoploidy (3x − 4x) in the root tips of one of the populations. Hybridization, polyploidy and dysploid variation may be the main factors associated with the chromosome number evolution of this species. FCM showed that 2C DNA contents vary from 0.87 to 2.02 pg, showing more than a 2-fold variation. The mean amount of 2C DNA/chromosome and the mean of monoploid genome size were not proportional to ploidy.
KW - dysploidy
KW - genome size
KW - Hypericum perforatum
KW - karyotype variability
KW - mixoploidy
KW - polyploidy
UR - http://www.scopus.com/inward/record.url?scp=85142616998&partnerID=8YFLogxK
U2 - 10.3390/plants11223068
DO - 10.3390/plants11223068
M3 - Article
C2 - 36432797
AN - SCOPUS:85142616998
VL - 11
JO - Plants
JF - Plants
SN - 2223-7747
IS - 22
M1 - 3068
ER -