Mitochondrial Respiration and Oxygen Tension

Daniel S Shaw, Karlia Meitha, Michael J Considine, Christine H Foyer

Research output: Chapter in Book/Conference paperChapter

Abstract

Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].

Original languageEnglish
Title of host publicationPlant Respiration and Internal Oxygen
Subtitle of host publicationmethods and protocols
EditorsKapuganti Jagadis Gupta
PublisherSpringer
Pages97-113
Number of pages17
Volume1670
ISBN (Electronic)9781493972920
ISBN (Print)9781493972913
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

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Respiration
Oxygen
Respiratory Transport
Electron Transport
Mitochondria

Cite this

Shaw, D. S., Meitha, K., Considine, M. J., & Foyer, C. H. (2017). Mitochondrial Respiration and Oxygen Tension. In K. J. Gupta (Ed.), Plant Respiration and Internal Oxygen: methods and protocols (Vol. 1670, pp. 97-113). (Methods in molecular biology (Clifton, N.J.)). Springer. https://doi.org/10.1007/978-1-4939-7292-0_11
Shaw, Daniel S ; Meitha, Karlia ; Considine, Michael J ; Foyer, Christine H. / Mitochondrial Respiration and Oxygen Tension. Plant Respiration and Internal Oxygen: methods and protocols. editor / Kapuganti Jagadis Gupta. Vol. 1670 Springer, 2017. pp. 97-113 (Methods in molecular biology (Clifton, N.J.)).
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Shaw, DS, Meitha, K, Considine, MJ & Foyer, CH 2017, Mitochondrial Respiration and Oxygen Tension. in KJ Gupta (ed.), Plant Respiration and Internal Oxygen: methods and protocols. vol. 1670, Methods in molecular biology (Clifton, N.J.), Springer, pp. 97-113. https://doi.org/10.1007/978-1-4939-7292-0_11

Mitochondrial Respiration and Oxygen Tension. / Shaw, Daniel S; Meitha, Karlia; Considine, Michael J; Foyer, Christine H.

Plant Respiration and Internal Oxygen: methods and protocols. ed. / Kapuganti Jagadis Gupta. Vol. 1670 Springer, 2017. p. 97-113 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Conference paperChapter

TY - CHAP

T1 - Mitochondrial Respiration and Oxygen Tension

AU - Shaw, Daniel S

AU - Meitha, Karlia

AU - Considine, Michael J

AU - Foyer, Christine H

PY - 2017

Y1 - 2017

N2 - Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].

AB - Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].

KW - Journal Article

U2 - 10.1007/978-1-4939-7292-0_11

DO - 10.1007/978-1-4939-7292-0_11

M3 - Chapter

SN - 9781493972913

VL - 1670

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 97

EP - 113

BT - Plant Respiration and Internal Oxygen

A2 - Gupta, Kapuganti Jagadis

PB - Springer

ER -

Shaw DS, Meitha K, Considine MJ, Foyer CH. Mitochondrial Respiration and Oxygen Tension. In Gupta KJ, editor, Plant Respiration and Internal Oxygen: methods and protocols. Vol. 1670. Springer. 2017. p. 97-113. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-7292-0_11