Mitochondrial Respiration and Oxygen Tension

Daniel S Shaw, Karlia Meitha, Michael J Considine, Christine H Foyer

Research output: Chapter in Book/Conference paperChapter

Abstract

Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].

Original languageEnglish
Title of host publicationPlant Respiration and Internal Oxygen
Subtitle of host publicationmethods and protocols
EditorsKapuganti Jagadis Gupta
PublisherSpringer
Pages97-113
Number of pages17
Volume1670
ISBN (Electronic)9781493972920
ISBN (Print)9781493972913
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745

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