Abstract
Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].
| Original language | English |
|---|---|
| Title of host publication | Plant Respiration and Internal Oxygen |
| Subtitle of host publication | methods and protocols |
| Editors | Kapuganti Jagadis Gupta |
| Publisher | Springer |
| Pages | 97-113 |
| Number of pages | 17 |
| Volume | 1670 |
| ISBN (Electronic) | 9781493972920 |
| ISBN (Print) | 9781493972913 |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in molecular biology (Clifton, N.J.) |
|---|---|
| Publisher | Humana Press |
| ISSN (Print) | 1064-3745 |
Fingerprint
Cite this
}
Mitochondrial Respiration and Oxygen Tension. / Shaw, Daniel S; Meitha, Karlia; Considine, Michael J; Foyer, Christine H.
Plant Respiration and Internal Oxygen: methods and protocols. ed. / Kapuganti Jagadis Gupta. Vol. 1670 Springer, 2017. p. 97-113 (Methods in molecular biology (Clifton, N.J.)).Research output: Chapter in Book/Conference paper › Chapter
TY - CHAP
T1 - Mitochondrial Respiration and Oxygen Tension
AU - Shaw, Daniel S
AU - Meitha, Karlia
AU - Considine, Michael J
AU - Foyer, Christine H
PY - 2017
Y1 - 2017
N2 - Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].
AB - Measurements of respiration and oxygen tension in plant organs allow a precise understanding of mitochondrial capacity and function within the context of cellular oxygen metabolism. Here we describe methods that can be routinely used for the isolation of intact mitochondria, and the determination of respiratory electron transport, together with techniques for in vivo determination of oxygen tension and measurement of respiration by both CO2 production and O2 consumption that enables calculation of the respiratory quotient [CO2]/[O2].
KW - Journal Article
U2 - 10.1007/978-1-4939-7292-0_11
DO - 10.1007/978-1-4939-7292-0_11
M3 - Chapter
SN - 9781493972913
VL - 1670
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 97
EP - 113
BT - Plant Respiration and Internal Oxygen
A2 - Gupta, Kapuganti Jagadis
PB - Springer
ER -