Mitochondrial complex II of plants: subunit composition, assembly, and function in respiration and signaling

Shaobai Huang, Hans-Peter Braun, Ryan Gawryluk, Andrew Millar

Research output: Contribution to journalReview article

Abstract

Complex II [succinate dehydrogenase (succinate-ubiquinone oxidoreductase); EC 1.3.5.1; SDH] is the only enzyme shared by both the electron transport chain and the tricarboxylic acid (TCA) cycle in mitochondria. Complex II in plants is considered unusual because of its accessory subunits (SDH5-SDH8), in addition to the catalytic subunits of SDH found in all eukaryotes (SDH1-SDH4). Here, we review compositional and phylogenetic analysis and biochemical dissection studies to both clarify the presence and propose a role for these subunits. We also consider the wider functional and phylogenetic evidence for SDH assembly factors and the reports from plants on the control of SDH1 flavination and SDH1-SDH2 interaction. Plant complex II has been shown to influence stomatal opening, the plant defense response and reactive oxygen species-dependent stress responses. Signaling molecules such as salicyclic acid (SA) and nitric oxide (NO) are also reported to interact with the ubiquinone (UQ) binding site of SDH, influencing signaling transduction in plants. Future directions for SDH research in plants and the specific roles of its different subunits and assembly factors are suggested, including the potential for reverse electron transport to explain the succinate-dependent production of reactive oxygen species in plants and new avenues to explore the evolution of plant mitochondrial complex II and its utility.

Significance Statement Mitochondrial complex II is the only component shared between the electron transport chain and the tricarboxylic acid (TCA) cycle, and it plays a key role in respiration, metabolism and signaling in stress and pathogen responses in plants.

Original languageEnglish
Pages (from-to)405-417
Number of pages13
JournalThe Plant Journal
Volume98
Issue number3
DOIs
Publication statusPublished - May 2019

Fingerprint

cell respiration
Respiration
succinate dehydrogenase (quinone)
Electron Transport Complex II
Electron Transport
tricarboxylic acid cycle
electron transport chain
Citric Acid Cycle
reactive oxygen species
Reactive Oxygen Species
ubiquinones
phylogeny
Ubiquinone
protein subunits
succinic acid
Succinic Acid
electron transfer
Eukaryota
nitric oxide
plant response

Cite this

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title = "Mitochondrial complex II of plants: subunit composition, assembly, and function in respiration and signaling",
abstract = "Complex II [succinate dehydrogenase (succinate-ubiquinone oxidoreductase); EC 1.3.5.1; SDH] is the only enzyme shared by both the electron transport chain and the tricarboxylic acid (TCA) cycle in mitochondria. Complex II in plants is considered unusual because of its accessory subunits (SDH5-SDH8), in addition to the catalytic subunits of SDH found in all eukaryotes (SDH1-SDH4). Here, we review compositional and phylogenetic analysis and biochemical dissection studies to both clarify the presence and propose a role for these subunits. We also consider the wider functional and phylogenetic evidence for SDH assembly factors and the reports from plants on the control of SDH1 flavination and SDH1-SDH2 interaction. Plant complex II has been shown to influence stomatal opening, the plant defense response and reactive oxygen species-dependent stress responses. Signaling molecules such as salicyclic acid (SA) and nitric oxide (NO) are also reported to interact with the ubiquinone (UQ) binding site of SDH, influencing signaling transduction in plants. Future directions for SDH research in plants and the specific roles of its different subunits and assembly factors are suggested, including the potential for reverse electron transport to explain the succinate-dependent production of reactive oxygen species in plants and new avenues to explore the evolution of plant mitochondrial complex II and its utility.Significance Statement Mitochondrial complex II is the only component shared between the electron transport chain and the tricarboxylic acid (TCA) cycle, and it plays a key role in respiration, metabolism and signaling in stress and pathogen responses in plants.",
author = "Shaobai Huang and Hans-Peter Braun and Ryan Gawryluk and Andrew Millar",
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Mitochondrial complex II of plants : subunit composition, assembly, and function in respiration and signaling. / Huang, Shaobai; Braun, Hans-Peter; Gawryluk, Ryan; Millar, Andrew.

In: The Plant Journal, Vol. 98, No. 3, 05.2019, p. 405-417.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Mitochondrial complex II of plants

T2 - subunit composition, assembly, and function in respiration and signaling

AU - Huang, Shaobai

AU - Braun, Hans-Peter

AU - Gawryluk, Ryan

AU - Millar, Andrew

PY - 2019/5

Y1 - 2019/5

N2 - Complex II [succinate dehydrogenase (succinate-ubiquinone oxidoreductase); EC 1.3.5.1; SDH] is the only enzyme shared by both the electron transport chain and the tricarboxylic acid (TCA) cycle in mitochondria. Complex II in plants is considered unusual because of its accessory subunits (SDH5-SDH8), in addition to the catalytic subunits of SDH found in all eukaryotes (SDH1-SDH4). Here, we review compositional and phylogenetic analysis and biochemical dissection studies to both clarify the presence and propose a role for these subunits. We also consider the wider functional and phylogenetic evidence for SDH assembly factors and the reports from plants on the control of SDH1 flavination and SDH1-SDH2 interaction. Plant complex II has been shown to influence stomatal opening, the plant defense response and reactive oxygen species-dependent stress responses. Signaling molecules such as salicyclic acid (SA) and nitric oxide (NO) are also reported to interact with the ubiquinone (UQ) binding site of SDH, influencing signaling transduction in plants. Future directions for SDH research in plants and the specific roles of its different subunits and assembly factors are suggested, including the potential for reverse electron transport to explain the succinate-dependent production of reactive oxygen species in plants and new avenues to explore the evolution of plant mitochondrial complex II and its utility.Significance Statement Mitochondrial complex II is the only component shared between the electron transport chain and the tricarboxylic acid (TCA) cycle, and it plays a key role in respiration, metabolism and signaling in stress and pathogen responses in plants.

AB - Complex II [succinate dehydrogenase (succinate-ubiquinone oxidoreductase); EC 1.3.5.1; SDH] is the only enzyme shared by both the electron transport chain and the tricarboxylic acid (TCA) cycle in mitochondria. Complex II in plants is considered unusual because of its accessory subunits (SDH5-SDH8), in addition to the catalytic subunits of SDH found in all eukaryotes (SDH1-SDH4). Here, we review compositional and phylogenetic analysis and biochemical dissection studies to both clarify the presence and propose a role for these subunits. We also consider the wider functional and phylogenetic evidence for SDH assembly factors and the reports from plants on the control of SDH1 flavination and SDH1-SDH2 interaction. Plant complex II has been shown to influence stomatal opening, the plant defense response and reactive oxygen species-dependent stress responses. Signaling molecules such as salicyclic acid (SA) and nitric oxide (NO) are also reported to interact with the ubiquinone (UQ) binding site of SDH, influencing signaling transduction in plants. Future directions for SDH research in plants and the specific roles of its different subunits and assembly factors are suggested, including the potential for reverse electron transport to explain the succinate-dependent production of reactive oxygen species in plants and new avenues to explore the evolution of plant mitochondrial complex II and its utility.Significance Statement Mitochondrial complex II is the only component shared between the electron transport chain and the tricarboxylic acid (TCA) cycle, and it plays a key role in respiration, metabolism and signaling in stress and pathogen responses in plants.

U2 - 10.1111/tpj.14227

DO - 10.1111/tpj.14227

M3 - Review article

VL - 98

SP - 405

EP - 417

JO - The Plant Journal

JF - The Plant Journal

SN - 0960-7412

IS - 3

ER -