miR-136-3p targets PTEN to regulate vascularization and bone formation and ameliorates alcohol-induced osteopenia

Yixuan Chen, Hongping Yu, Daoyu Zhu, Pei Liu, Junhui Yin, Delin Liu, Minghao Zheng, Junjie Gao, Changqing Zhang, Youshui Gao

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Alcohol consumption is regarded as one of the leading risk factors for secondary osteopenia. Coupled angiogenesis and osteogenesis via distinct type-H vessels orchestrates subtle biological processes of bone homeostasis. The dysfunction of angiogenesis and osteogenesis contributes to decreased bone mass during the development of osteopenia. Herein, we identified microRNA-136-3p was remarkedly downregulated in the mouse model of alcohol-induced osteopenia. Following the alcohol administration, downregulated microRNA-136-3p significantly suppressed vascularization and osteogenic differentiation in human umbilical vein endothelial cells (HUVECs) and bone mesenchymal stem cells (BMSCs), respectively. Furthermore, microRNA-136-3p could target phosphatase and tensin homolog deleted on chromosome ten (PTEN) in both HUVECs and BMSCs, thus substantially modulating the capacity of vessel formation and osteogenic differentiation. In the mouse model, microRNA-136-3p Agomir ameliorated alcohol-induced osteopenia, with the concomitant restoration of bone mass and type-H vessel formation. For the first time, this study demonstrated the pivotal role of microRNA-136-3p/PTEN axis in regulations of vascularization and bone formation, which might become the potential therapeutic target of alcohol-induced bone loss.

Original languageEnglish
Pages (from-to)5348-5362
Number of pages15
JournalFASEB Journal
Volume34
Issue number4
DOIs
Publication statusPublished - 1 Apr 2020

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