TY - JOUR
T1 - Minimizing artifactual elevation of lipid peroxidation products (F 2-isoprostanes) in plasma during collection and storage
AU - Barden, Anne
AU - Mas, Emilie
AU - Croft, Kevin
AU - Phillips, Michael
AU - Mori, Trevor
PY - 2013
Y1 - 2013
N2 - F2-isoprostanes (F2-IsoP's) are reliable measures of in vivo lipid oxidation, but care is required to prevent artifactual elevation. We examined the effects of blood collection and storage on plasma F 2-IsoP's. Blood was collected into EDTA/butylated hydroxytoluene/reduced glutathione (EDTA/BHT/GSH) or EDTA, at 4 C or room temperature. Plasma was stored at -20 or -80 C for 1 or 6 months before F 2-IsoP's were assayed by GC-MS. The temperature of blood collection did not affect F2-IsoP's. However, storage at -20 C or collection into EDTA resulted in significant increases in F2-IsoP's. Blood collection into EDTA/BHT/GSH and storage at -80 C minimizes artifactual elevation of plasma F2-IsoP's. © 2014 Elsevier Inc. All rights reserved.
AB - F2-isoprostanes (F2-IsoP's) are reliable measures of in vivo lipid oxidation, but care is required to prevent artifactual elevation. We examined the effects of blood collection and storage on plasma F 2-IsoP's. Blood was collected into EDTA/butylated hydroxytoluene/reduced glutathione (EDTA/BHT/GSH) or EDTA, at 4 C or room temperature. Plasma was stored at -20 or -80 C for 1 or 6 months before F 2-IsoP's were assayed by GC-MS. The temperature of blood collection did not affect F2-IsoP's. However, storage at -20 C or collection into EDTA resulted in significant increases in F2-IsoP's. Blood collection into EDTA/BHT/GSH and storage at -80 C minimizes artifactual elevation of plasma F2-IsoP's. © 2014 Elsevier Inc. All rights reserved.
UR - https://www.scopus.com/pages/publications/84892764858
U2 - 10.1016/j.ab.2013.12.030
DO - 10.1016/j.ab.2013.12.030
M3 - Article
C2 - 24380789
SN - 0003-2697
VL - 449
SP - 129
EP - 131
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -