Millimeter wave radiation activates leech nociceptors via TRPV1-like receptor sensitization

Research output: Contribution to journalArticle

Abstract

There is evidence that millimeter waves (MMWs) can have an impact on cellular function, including neurons. Earlier in vitro studies have shown that exposure levels well below the recommended safe limit of 1 mW/cm 2 cause changes in the action potential (AP) firing rate, resting potential, and AP pulse shape of sensory neurons in leech preparations as well as alter neuronal properties in rat cortical brain slices; these effects differ from changes induced by direct heating. In this article, we compare the responses of thermosensitive primary nociceptors of the medicinal leech under thermal heating and MMW irradiation (80–170 mW/cm 2 at 60 GHz). The results show that MMW exposure causes an almost twofold decrease in the threshold for activation of the AP compared with thermal heating (3.9 ± 0.4 vs. 8.3 ± 0.4 mV, respectively). Our analysis suggests that MMWs-mediated threshold alterations are not caused by the enhancement of voltage-gated sodium and potassium conductance. We propose that the reduction in AP threshold can be attributed to the sensitization of the transient receptor potential vanilloid 1-like receptor in the leech nociceptor. In silico modeling supported our experimental findings. Our results provide evidence that MMW exposure stimulates specific receptor responses that differ from direct thermal heating, fostering the need for additional studies.

Original languageEnglish
Pages (from-to)2331-2345
Number of pages15
JournalBiophysical Journal
DOIs
Publication statusE-pub ahead of print - 25 Apr 2019

Fingerprint

Leeches
Nociceptors
Heating
Action Potentials
Radiation
Hot Temperature
Foster Home Care
Sensory Receptor Cells
Computer Simulation
Membrane Potentials
Potassium
Sodium
Neurons
TRPV1 receptor
Brain

Cite this

@article{7012a4c9f9b44a11b808f57e429357ac,
title = "Millimeter wave radiation activates leech nociceptors via TRPV1-like receptor sensitization",
abstract = "There is evidence that millimeter waves (MMWs) can have an impact on cellular function, including neurons. Earlier in vitro studies have shown that exposure levels well below the recommended safe limit of 1 mW/cm 2 cause changes in the action potential (AP) firing rate, resting potential, and AP pulse shape of sensory neurons in leech preparations as well as alter neuronal properties in rat cortical brain slices; these effects differ from changes induced by direct heating. In this article, we compare the responses of thermosensitive primary nociceptors of the medicinal leech under thermal heating and MMW irradiation (80–170 mW/cm 2 at 60 GHz). The results show that MMW exposure causes an almost twofold decrease in the threshold for activation of the AP compared with thermal heating (3.9 ± 0.4 vs. 8.3 ± 0.4 mV, respectively). Our analysis suggests that MMWs-mediated threshold alterations are not caused by the enhancement of voltage-gated sodium and potassium conductance. We propose that the reduction in AP threshold can be attributed to the sensitization of the transient receptor potential vanilloid 1-like receptor in the leech nociceptor. In silico modeling supported our experimental findings. Our results provide evidence that MMW exposure stimulates specific receptor responses that differ from direct thermal heating, fostering the need for additional studies.",
author = "Sergii Romanenko and Harvey, {Alan R.} and Livia Hool and Shuting Fan and Wallace, {Vincent P.}",
year = "2019",
month = "4",
day = "25",
doi = "10.1016/j.bpj.2019.04.021",
language = "English",
pages = "2331--2345",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Cell Press",

}

TY - JOUR

T1 - Millimeter wave radiation activates leech nociceptors via TRPV1-like receptor sensitization

AU - Romanenko, Sergii

AU - Harvey, Alan R.

AU - Hool, Livia

AU - Fan, Shuting

AU - Wallace, Vincent P.

PY - 2019/4/25

Y1 - 2019/4/25

N2 - There is evidence that millimeter waves (MMWs) can have an impact on cellular function, including neurons. Earlier in vitro studies have shown that exposure levels well below the recommended safe limit of 1 mW/cm 2 cause changes in the action potential (AP) firing rate, resting potential, and AP pulse shape of sensory neurons in leech preparations as well as alter neuronal properties in rat cortical brain slices; these effects differ from changes induced by direct heating. In this article, we compare the responses of thermosensitive primary nociceptors of the medicinal leech under thermal heating and MMW irradiation (80–170 mW/cm 2 at 60 GHz). The results show that MMW exposure causes an almost twofold decrease in the threshold for activation of the AP compared with thermal heating (3.9 ± 0.4 vs. 8.3 ± 0.4 mV, respectively). Our analysis suggests that MMWs-mediated threshold alterations are not caused by the enhancement of voltage-gated sodium and potassium conductance. We propose that the reduction in AP threshold can be attributed to the sensitization of the transient receptor potential vanilloid 1-like receptor in the leech nociceptor. In silico modeling supported our experimental findings. Our results provide evidence that MMW exposure stimulates specific receptor responses that differ from direct thermal heating, fostering the need for additional studies.

AB - There is evidence that millimeter waves (MMWs) can have an impact on cellular function, including neurons. Earlier in vitro studies have shown that exposure levels well below the recommended safe limit of 1 mW/cm 2 cause changes in the action potential (AP) firing rate, resting potential, and AP pulse shape of sensory neurons in leech preparations as well as alter neuronal properties in rat cortical brain slices; these effects differ from changes induced by direct heating. In this article, we compare the responses of thermosensitive primary nociceptors of the medicinal leech under thermal heating and MMW irradiation (80–170 mW/cm 2 at 60 GHz). The results show that MMW exposure causes an almost twofold decrease in the threshold for activation of the AP compared with thermal heating (3.9 ± 0.4 vs. 8.3 ± 0.4 mV, respectively). Our analysis suggests that MMWs-mediated threshold alterations are not caused by the enhancement of voltage-gated sodium and potassium conductance. We propose that the reduction in AP threshold can be attributed to the sensitization of the transient receptor potential vanilloid 1-like receptor in the leech nociceptor. In silico modeling supported our experimental findings. Our results provide evidence that MMW exposure stimulates specific receptor responses that differ from direct thermal heating, fostering the need for additional studies.

UR - http://www.scopus.com/inward/record.url?scp=85065589501&partnerID=8YFLogxK

U2 - 10.1016/j.bpj.2019.04.021

DO - 10.1016/j.bpj.2019.04.021

M3 - Article

SP - 2331

EP - 2345

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

ER -