Milk antibodies for the treatment of gastroenteritis in developing countries

Mohammad Ahmad

    Research output: ThesisMaster's Thesis

    189 Downloads (Pure)

    Abstract

    Gastroenteritis caused by bacterial and viral pathogens has been the cause of death to more than 1.7 million children less than five years of age in developing countries. Rotavirus and Enterotoxigenic Escherichia.coli (ETEC) are among the main causes of gastroenteritis and diarrhoea that causes dehydration, and even death. Using immunotherapy against gastroenteritis is not novel. Previous works succeeded to treat gastroenteritis and diarrhoea using immunotherapy. The idea that dams produce antibodies against different types of antigens, and these antibodies are transferred to milk as passive immunity to offspring, has been implemented in this project to produce polyclonal antibodies against ETEC O78:h11, NL053 (O6:h16) and rotavirus SA11 in the milk of sheep. The levels of these polyclonal antibodies were measured in milk and serum using enzyme-linked Immunosorbent assay. Fifty Balb/c mice were used in this study. The mice were inoculated with ETEC O78:h11 and then challenged using milk enriched with antibodies against ETEC O78:h11 or against enterotoxigenic Escherichia.coli strain O6:H16 and rotavirus strain SA11 to measure the effectiveness of antibodies in neutralising the pathogens of interest. Heat stable enterotoxin was measured using the commercial kit COLIST (Denka Seiken, Japan) to assess the effectiveness of milk in neutralising ETEC O78:h11. Survival of the antibodies in the intestine of Balb/c mice was studied through measuring the level of antibodies against ETEC strain O78:h11, O6:H16 and rotavirus SA11 in the faecal samples during milk treatment period and after weaning. Results showed that antibody level in serum and milk increased after three boosts of each vaccine. The antibody level in the intestine of mice was high during milk treatment and sharply decreased after weaning. Heat stable enterotoxin test was negative for the treated groups 1 and 3 whereas it was positive for group 2. This group was given the milk without antibodies.
    Original languageEnglish
    QualificationMasters
    Publication statusUnpublished - 2010

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