Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy

Timothy Hillman, Sergey Alexandrov, Thomas Gutzler, David Sampson

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view. (c) 2006 Optical Society of America.
    Original languageEnglish
    Pages (from-to)11088-11094
    JournalOptics Express
    Volume14
    Issue number23
    DOIs
    Publication statusPublished - 2006

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