Microsatellites in the mitochondrial genome of Phytophthora cinnamomi failed to provide highly polymorphic markers for population genetics

Mark P. Dobrowolski, I. C. Tommerup, Philip A. O'Brien

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Microsatellites were evaluated as genetic markers for the mitochondrial genome (mtDNA) of Phytophthora cinnamomi for population studies. Two (A)(n) microsatellite loci were cloned from the mtDNA of P. cinnamomi. Amplification products from these loci showed little polymorphism among Phytophthora isolates due to their location in coding regions of mtDNA. A further three (A)(n) microsatellite loci obtained from the complete mtDNA sequence of P. infestans were also not highly polymorphic, although located in non-coding mtDNA. The presence of the (A)(n) microsatellites was not conserved in the genus Phytophthora. Unlike those of the chloroplast genome of plants, (A)(n) microsatellite loci of mtDNA do not have potential as highly polymorphic markers in Phytophthora.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalFEMS Microbiology Letters
Volume163
Issue number2
DOIs
Publication statusPublished - 15 Jun 1998
Externally publishedYes

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