Microsatellite diversity and crossover regions within homozygous and heterozygous SLA haplotypes of different pig breeds

A. Ando, H. Uenishi, H. Kawata, M. Tanaka-Matsuda, A. Shigenari, L. Flori, P. Chardon, J. Lunney, Jerzy Kulski, H. Inoko

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    14 Citations (Scopus)


    Our aim was to investigate microsatellite (MS) diversity and find crossover regions at 42 polymorphic MS loci in the swine leukocyte antigen (SLA) genomic region of 72 pigs with different well-defined homozygous and heterozygous SLA haplotypes. We analyzed the genetic polymorphisms of 42 MS markers in 23 SLA homozygous–heterozygous, common pig breeds with 12 SLA serological haplotypes and 49 National Institutes of Health (NIH) and Clawn homozygous–heterozygous miniature pigs with nine SLA serological or genotyped haplotypes including four recombinant haplotypes. In comparing the same and different haplotypes, both haplospecific patterns and allelic variations were observed at the MS loci. Some of the shared haplotype blocks extended over 2 Mb suggesting the existence of strong linkage disequilibrium (LD) in the entire SLA region. Crossover regions were easily defined by the MS markers within the class I and/or III region in the NIH and Clawn recombinant haplotypes. The present haplotype comparison shows that our set of MS markers provides a fast and cost-efficient alternative, or complementary, method to the serological or sequence-based determination of the SLA alleles for the characterization of SLA haplotypes and/or the crossover regions between different haplotypes.Keywords Swine major histocompatibility antigen - Microsatellite marker - Polymorphism - Homozygote - CrossoverImages **Fulltext PreviewImage of the first page of the fulltext documentShare this Item * email * citeulike * Connotea * DeliciousFrequently asked questions General info on journals and books Send us your feedback Impressum Contact us© Springer, Part of Springer Science+Business Media Privacy, Disclaimer, Terms & Conditions, and Copyright Info * Not Logged In * Recognized As: o The University of Western Australia (565-28-928) o GO8 Universities Australia (647-04-468) * Remote Address: * Server: MPWEB41 * HTTP User Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv: Gecko/20091221 Firefox/3.5.7 (.NET CLR 3.5.30729) * * closeUsernamePasswordRemember MeForgot your Password? Register Now Log In via Shibboleth or Athens closeImmunogeneticsRapid assignment of swine leukocyte antigen haplotypes in pedigreed herds using a polymerase chain reaction-based assay
    Original languageEnglish
    Pages (from-to)399-407
    Issue number7
    Publication statusPublished - 2008


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