Microbial and algal alginate gelation characterized by magnetic resonance

H.T. Fabich, Sarah Vogt, M.L. Sherick, J.D. Seymour, J.R. Brown, M.J. Franklin, S.L. Codd

    Research output: Contribution to journalArticle

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    Abstract

    Advanced magnetic resonance (MR) relaxation and diffusion correlation measurements and imaging provide a means to non-invasively monitor gelation for biotechnology applications. In this study, MR is used to characterize physical gelation of three alginates with distinct chemical structures; an algal alginate, which is not O-acetylated but contains poly guluronate (G) blocks, bacterial alginate from Pseudomonas aeruginosa, which does not have poly-G blocks, but is O-acetylated at the C2 and/or C3 of the mannuronate residues, and alginate from a P. aeruginosa mutant that lacks O-acetyl groups. The MR data indicate that diffusion-reaction front gelation with Ca2+ ions generates gels of different bulk homogeneities dependent on the alginate structure. Shorter spin-spin T2 magnetic relaxation times in the alginate gels that lack O-acetyl groups indicate stronger molecular interaction between the water and biopolymer. The data characterize gel differences over a hierarchy of scales from molecular to system size. © 2012 Elsevier B.V.
    Original languageEnglish
    Pages (from-to)320-327
    JournalJournal of Biotechnology
    Volume161
    Issue number3
    DOIs
    Publication statusPublished - 2012

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    Magnetic Resonance Spectroscopy
    Poly G
    Gels
    Pseudomonas aeruginosa
    Alginates
    Biopolymers
    Biotechnology
    alginic acid
    Ions
    Water

    Cite this

    Fabich, H. T., Vogt, S., Sherick, M. L., Seymour, J. D., Brown, J. R., Franklin, M. J., & Codd, S. L. (2012). Microbial and algal alginate gelation characterized by magnetic resonance. Journal of Biotechnology, 161(3), 320-327. https://doi.org/10.1016/j.jbiotec.2012.04.016
    Fabich, H.T. ; Vogt, Sarah ; Sherick, M.L. ; Seymour, J.D. ; Brown, J.R. ; Franklin, M.J. ; Codd, S.L. / Microbial and algal alginate gelation characterized by magnetic resonance. In: Journal of Biotechnology. 2012 ; Vol. 161, No. 3. pp. 320-327.
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    abstract = "Advanced magnetic resonance (MR) relaxation and diffusion correlation measurements and imaging provide a means to non-invasively monitor gelation for biotechnology applications. In this study, MR is used to characterize physical gelation of three alginates with distinct chemical structures; an algal alginate, which is not O-acetylated but contains poly guluronate (G) blocks, bacterial alginate from Pseudomonas aeruginosa, which does not have poly-G blocks, but is O-acetylated at the C2 and/or C3 of the mannuronate residues, and alginate from a P. aeruginosa mutant that lacks O-acetyl groups. The MR data indicate that diffusion-reaction front gelation with Ca2+ ions generates gels of different bulk homogeneities dependent on the alginate structure. Shorter spin-spin T2 magnetic relaxation times in the alginate gels that lack O-acetyl groups indicate stronger molecular interaction between the water and biopolymer. The data characterize gel differences over a hierarchy of scales from molecular to system size. {\circledC} 2012 Elsevier B.V.",
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    Fabich, HT, Vogt, S, Sherick, ML, Seymour, JD, Brown, JR, Franklin, MJ & Codd, SL 2012, 'Microbial and algal alginate gelation characterized by magnetic resonance' Journal of Biotechnology, vol. 161, no. 3, pp. 320-327. https://doi.org/10.1016/j.jbiotec.2012.04.016

    Microbial and algal alginate gelation characterized by magnetic resonance. / Fabich, H.T.; Vogt, Sarah; Sherick, M.L.; Seymour, J.D.; Brown, J.R.; Franklin, M.J.; Codd, S.L.

    In: Journal of Biotechnology, Vol. 161, No. 3, 2012, p. 320-327.

    Research output: Contribution to journalArticle

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    AU - Vogt, Sarah

    AU - Sherick, M.L.

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