Method for measuring a comprehensive energy budget in a proliferating cell system over multiple cell cycles

M. Guppy, S-E. Kong, X. Niu, S. Busfield, Peter Klinken

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19 Citations (Scopus)

Abstract

Isolated cell systems are now being used very effectively to study a range of important biochemical questions, but their energy metabolism has never been comprehensively investigated. We have developed a system, using J2E cells, which enables us to measure total ATP turnover and the contribution of various fuels and pathways to this total in a dynamic, proliferating preparation. Cells are cultured in 500 mi airtight glass containers which enables (1) the measurement of oxygen consumption, (2) the collection and measurement of (CO2)-C-14 production from labelled fuels, and (3) the measurement of metabolite utilization and production. Data on cell numbers are then used to produce a curve of cell number vs. time, the area under which (cell numbers hour) is used as a base by which all measurements and experiments are compared. To our knowledge this is the first time a comprehensive energy budget has been measured in a proliferating cell system over a period that covers multiple cell cycles. (C) 1997 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)1-7
JournalJournal of Cellular Physiology
Volume170
DOIs
Publication statusPublished - 1997

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