Methacholine responsiveness in mice from 2 to 8 wk of age

E.M. Bozanich, T.Z. Janosi, R.A. Collins, C. Thamrin, D.J. Turner, Z. Hantos, Peter Sly

Research output: Contribution to journalArticle

16 Citations (Scopus)


Many chronic human lung diseases have their origin in early childhood, yet most murine models used to study them utilize adult mice. An important component of the asthma phenotype is exaggerated airway responses, frequently modelled by methacholine ( MCh) challenge. The present study was undertaken to characterize MCh responses in mice from 2 to 8 wk of age measuring absolute lung volume and volume-corrected respiratory mechanics as outcome variables. Female BALB/c mice aged 2, 3, 4, 6, and 8 wk were studied during cumulative intravenous MCh challenge. Following each MCh dose, absolute lung volume was measured plethysmographically at functional residual volume and during a slow inflation to 20-hPa transrespiratory pressure. Respiratory system impedance was measured continuously during the inflation maneuver and partitioned into airway and constant-phase parenchymal components by model fitting. Volume-corrected ( specific) estimates of respiratory mechanics were calculated. Intravenous MCh challenge induced a predominantly airway response with no evidence of airway closure in any age group. No changes in functional residual volume were seen in mice of any age during the MCh challenge. The specific airway resistance MCh dose response curves did not show significant differences between the age groups. The results from the present study do not show systematic differences in MCh responsiveness in mice from 2 to 8 wk of age.
Original languageEnglish
Pages (from-to)542-46
JournalJournal of Applied Physiology
Issue number2
Publication statusPublished - 2007

Fingerprint Dive into the research topics of 'Methacholine responsiveness in mice from 2 to 8 wk of age'. Together they form a unique fingerprint.

Cite this