Metabolic activation of aromatic amines by human pancreas

K.E. Anderson, G.J. Hammons, F.F. Kadlubar, J.D. Potter, K.R. Kaderlik, Kenneth Ilett, R.F. Minchin, C.H. Teitel, H-C. Chou, M.V. Martin, F.P. Guengerich, G.W. Barone, N.P. Lang, L.A. Peterson

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Abstract

Epidemiologic studies have suggested that aromatic amines (and nitroaromatic hydrocarbons) may be carcinogenic for human pancreas, Pancreatic tissues from 29 organ donors (13 smokers, 16 non-smokers) were examined for their ability to metabolize aromatic amines and other carcinogens, Microsomes showed no activity for cytochrome P450 (P450) 1A2-dependent N-oxidation of 4-aminobiphenyl (ABP) or for the following activities (and associated P450s): aminopyrine N-demethylation and ethylmorphine N-demethylation (P450 3A4); ethoxyresorufin O-deethylation (P450 1A1) and pentoxyresorufin O-dealkylation (P450 2B6); p-nitrophenol hydroxylation and N-nitrosodimethylamine N-demethylation (P450 2E1); lauric acid omega-hydroxylation (P450 4A1); and 4-(methylnitrosamino)-1-(3-pyridyl-1-butanol) (NNAL) and 4-(methylnitrosamino)1-(3-pyridyl)-1-butanone (NNK) alpha-oxidation (P450 1A2, 2A6, 2D6). Antibodies were used to examine microsomal levels of P450 1A2, 2A6, 2C8/9/18/19, 2E1, 2D6, and 3A3/ 4/5/7 and epoxide hydrolase. Immunoblots detected only epoxide hydrolase at low levels; P450 levels were
Original languageEnglish
Pages (from-to)1085-1092
JournalCarcinogenesis
Volume18
Issue number5
DOIs
Publication statusPublished - 1997

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    Anderson, K. E., Hammons, G. J., Kadlubar, F. F., Potter, J. D., Kaderlik, K. R., Ilett, K., ... Peterson, L. A. (1997). Metabolic activation of aromatic amines by human pancreas. Carcinogenesis, 18(5), 1085-1092. https://doi.org/10.1093/carcin/18.5.1085