Membrane-Mediated Protein–Protein Interactions of Cholesterol Side-Chain Cleavage Cytochrome P450 with its Associated Electron Transport Proteins

C. Kubeil, J.C.I. Yeung, Robert C. Tuckey, R.J. Rodgers, L.L. Martin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Cytochrome P450scc (P450scc or CYP11A1) catalyses the first enzymatic step of steroid biosynthesis, the cleavage of the side chain of cholesterol to produce pregnenolone in the mitochondrion. The activity of P450scc is dependent upon electron delivery from NADPH-dependent adrenodoxin reductase (AdR), via adrenodoxin (Adx), to the P450scc. However, despite the structural and kinetic data that supports the mechanism by which Adx shuttles electrons one at a time between AdR and the P450scc, there are limited data available on the influence of the lipid membrane on these essential interactions. In this paper, the protein–membrane interactions between P450scc and its redox partners were examined on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes containing cholesterol (20 %), using a quartz crystal microbalance with dissipation monitoring. P450scc showed strong binding to these membranes, whereas AdR and Adx both showed weaker association. If pre-mixed, all three proteins bound independently to the membrane layer in a distinctive two-stage process, as observed by frequency changes upon binding. Concomitant changes in the dissipation revealed specific protein–protein interaction occurs upon reaching a critical concentration of proteins in the membrane layer. These changes were specific for the binding of the three pre-mixed proteins and were not observed for a binary mixture of P450 and Adx, or sequential binding of the three proteins. A simple model was developed for the binding of all three proteins in a 1:1:1 mixture to the membrane and reproduces the experimental data describing the interaction of P450scc with the other proteins (AdR and Adx) after initial binding of the individual proteins. Thus, we conclude that the lipid membrane assists in the assembly of electron transport proteins and the activity of P450scc by providing a surface for the localised concentration of proteins, enabling them to act together as a metabolon.

Original languageEnglish
Pages (from-to)995-1002
JournalChemPlusChem
Volume81
Issue number9
Early online date22 Jul 2016
DOIs
Publication statusPublished - Sep 2016

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Adrenodoxin
Cytochrome P-450 Enzyme System
Ferredoxin-NADP Reductase
Carrier Proteins
Cholesterol
Membranes
Cholesterol Side-Chain Cleavage Enzyme
Proteins
Membrane Lipids
Pregnenolone
Mitochondria
Phosphorylcholine
Electrons
Quartz crystal microbalances
Biosynthesis
Binary mixtures
Electron Transport
NADP
Steroids
Association reactions

Cite this

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title = "Membrane-Mediated Protein–Protein Interactions of Cholesterol Side-Chain Cleavage Cytochrome P450 with its Associated Electron Transport Proteins",
abstract = "{\circledC} 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Cytochrome P450scc (P450scc or CYP11A1) catalyses the first enzymatic step of steroid biosynthesis, the cleavage of the side chain of cholesterol to produce pregnenolone in the mitochondrion. The activity of P450scc is dependent upon electron delivery from NADPH-dependent adrenodoxin reductase (AdR), via adrenodoxin (Adx), to the P450scc. However, despite the structural and kinetic data that supports the mechanism by which Adx shuttles electrons one at a time between AdR and the P450scc, there are limited data available on the influence of the lipid membrane on these essential interactions. In this paper, the protein–membrane interactions between P450scc and its redox partners were examined on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes containing cholesterol (20 {\%}), using a quartz crystal microbalance with dissipation monitoring. P450scc showed strong binding to these membranes, whereas AdR and Adx both showed weaker association. If pre-mixed, all three proteins bound independently to the membrane layer in a distinctive two-stage process, as observed by frequency changes upon binding. Concomitant changes in the dissipation revealed specific protein–protein interaction occurs upon reaching a critical concentration of proteins in the membrane layer. These changes were specific for the binding of the three pre-mixed proteins and were not observed for a binary mixture of P450 and Adx, or sequential binding of the three proteins. A simple model was developed for the binding of all three proteins in a 1:1:1 mixture to the membrane and reproduces the experimental data describing the interaction of P450scc with the other proteins (AdR and Adx) after initial binding of the individual proteins. Thus, we conclude that the lipid membrane assists in the assembly of electron transport proteins and the activity of P450scc by providing a surface for the localised concentration of proteins, enabling them to act together as a metabolon.",
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Membrane-Mediated Protein–Protein Interactions of Cholesterol Side-Chain Cleavage Cytochrome P450 with its Associated Electron Transport Proteins. / Kubeil, C.; Yeung, J.C.I.; Tuckey, Robert C.; Rodgers, R.J.; Martin, L.L.

In: ChemPlusChem, Vol. 81, No. 9, 09.2016, p. 995-1002.

Research output: Contribution to journalArticle

TY - JOUR

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N2 - © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Cytochrome P450scc (P450scc or CYP11A1) catalyses the first enzymatic step of steroid biosynthesis, the cleavage of the side chain of cholesterol to produce pregnenolone in the mitochondrion. The activity of P450scc is dependent upon electron delivery from NADPH-dependent adrenodoxin reductase (AdR), via adrenodoxin (Adx), to the P450scc. However, despite the structural and kinetic data that supports the mechanism by which Adx shuttles electrons one at a time between AdR and the P450scc, there are limited data available on the influence of the lipid membrane on these essential interactions. In this paper, the protein–membrane interactions between P450scc and its redox partners were examined on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes containing cholesterol (20 %), using a quartz crystal microbalance with dissipation monitoring. P450scc showed strong binding to these membranes, whereas AdR and Adx both showed weaker association. If pre-mixed, all three proteins bound independently to the membrane layer in a distinctive two-stage process, as observed by frequency changes upon binding. Concomitant changes in the dissipation revealed specific protein–protein interaction occurs upon reaching a critical concentration of proteins in the membrane layer. These changes were specific for the binding of the three pre-mixed proteins and were not observed for a binary mixture of P450 and Adx, or sequential binding of the three proteins. A simple model was developed for the binding of all three proteins in a 1:1:1 mixture to the membrane and reproduces the experimental data describing the interaction of P450scc with the other proteins (AdR and Adx) after initial binding of the individual proteins. Thus, we conclude that the lipid membrane assists in the assembly of electron transport proteins and the activity of P450scc by providing a surface for the localised concentration of proteins, enabling them to act together as a metabolon.

AB - © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Cytochrome P450scc (P450scc or CYP11A1) catalyses the first enzymatic step of steroid biosynthesis, the cleavage of the side chain of cholesterol to produce pregnenolone in the mitochondrion. The activity of P450scc is dependent upon electron delivery from NADPH-dependent adrenodoxin reductase (AdR), via adrenodoxin (Adx), to the P450scc. However, despite the structural and kinetic data that supports the mechanism by which Adx shuttles electrons one at a time between AdR and the P450scc, there are limited data available on the influence of the lipid membrane on these essential interactions. In this paper, the protein–membrane interactions between P450scc and its redox partners were examined on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes containing cholesterol (20 %), using a quartz crystal microbalance with dissipation monitoring. P450scc showed strong binding to these membranes, whereas AdR and Adx both showed weaker association. If pre-mixed, all three proteins bound independently to the membrane layer in a distinctive two-stage process, as observed by frequency changes upon binding. Concomitant changes in the dissipation revealed specific protein–protein interaction occurs upon reaching a critical concentration of proteins in the membrane layer. These changes were specific for the binding of the three pre-mixed proteins and were not observed for a binary mixture of P450 and Adx, or sequential binding of the three proteins. A simple model was developed for the binding of all three proteins in a 1:1:1 mixture to the membrane and reproduces the experimental data describing the interaction of P450scc with the other proteins (AdR and Adx) after initial binding of the individual proteins. Thus, we conclude that the lipid membrane assists in the assembly of electron transport proteins and the activity of P450scc by providing a surface for the localised concentration of proteins, enabling them to act together as a metabolon.

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