Melanocyte repopulation in full-thickness wounds using a cell spray apparatus

F. A. Navarro, M. L. Stoner, H. B. Lee, C. S. Park, F. M. Wood, D. P. Orgill

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Melanocyte restoration is critical in reconstituting skin color. We developed a spotted (pie-bald) pig wound model to study methods of restoring melanocytes to the epidermis. Paired, full-thickness, porcine wounds were covered with nonpigmented, fully expanded, 3:1 meshed, split-thickness skin grafts and were sprayed with an epidermal cell suspension. The suspensions were highly pigmented skin (HPS) cell isolates for half of the wounds (n = 16) and nonpigmented skin (NPS) cell isolates for the remaining wounds (n = 16). Histologic sections showed 6.0 ± 3.0 and 15 ± 4.0 pigmented melanocytes per high-power field on days 8 and 20 in HPS-treated wounds and no pigmented melanocytes in NPS-treated wounds. Melanin pigment was dispersed in all layers of the epithelium for the HPS group on day 20 compared with a lack of melanin pigment observed in the NPS group. Cell spraying may provide a clinical method to restore color to skin; further work is needed to control the expression of melanin.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalJournal of Burn Care and Rehabilitation
Volume22
Issue number1
DOIs
Publication statusPublished - 1 Jan 2001

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